Rabbit SRSF7 Polyclonal Antibody | anti-SRSF7 antibody

SRSF7 (N103) Polyclonal Antibody

Cat. Num. AAA3004477

• Gene Names: SRSF7; 9G8; AAG3; SFRS7
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SRSF7; Polyclonal Antibody; SRSF7 (N103) Polyclonal Antibody; Serine/arginine-rich splicing factor 7; Splicing factor 9G8; Splicing factor; arginine/serine-rich 7; SFRS7; anti-SRSF7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SRSF7 (N103) polyclonal antibody detects endogenous levels of SRSF7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 238

• Applicable Applications for anti-SRSF7 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 71-122 of Human SRSF7.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of SRSF7 (N103) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-SRSF7 antibody

Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF. The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine (RS)-rich domain. SR factors are essential for constitutive splicing and also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport.

NCBI and Uniprot Product Information

• NCBI GI #: 72534660
• NCBI GeneID: 6432
• NCBI Accession #: NP_001026854.1
• NCBI GenBank Nucleotide #: NP_001026854.1
• UniProt Accession #: Q16629
• Molecular Weight: ~ 35kDa
• NCBI Official Full Name: serine/arginine-rich splicing factor 7 isoform 1
• NCBI Official Synonym Full Names: serine and arginine rich splicing factor 7
• NCBI Official Symbol: SRSF7
• NCBI Official Synonym Symbols: 9G8; AAG3; SFRS7
• NCBI Protein Information: serine/arginine-rich splicing factor 7
• UniProt Protein Name: Serine/arginine-rich splicing factor 7
• Protein Family: Serine/arginine-rich splicing factor
• UniProt Gene Name: SRSF7
• UniProt Synonym Gene Names: SFRS7
• UniProt Entry Name: SRSF7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an N-terminal RNA recognition motif (RRM) for binding RNA and a C-terminal RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2018]

Uniprot Description

SFRS7: Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 2p22.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription