Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: K562 cells, Primary antibody: 861699(GAGE12I Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Rabbit anti-Human GAGE12I Polyclonal Antibody | anti-GAGE12I antibody
GAGE12I Rabbit pAb
Gene Names
GAGE12I; AL4; CT4.7; GAGE7B; GAGE-7B
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
GAGE12I; Polyclonal Antibody; GAGE12I Rabbit pAb; AL4; CT4.7; GAGE7B; GAGE-7B; anti-GAGE12I antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-GAGE12I antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
Immunogen
Synthetic peptide of human GAGE12I
Conjugation
Unconjugated
Molecular Function
This gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The antigenic peptide YYWPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes.
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: K562 cells, Primary antibody: 861699(GAGE12I Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: K562 cells, Primary antibody: 861699(GAGE12I Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 861699(GAGE12I Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 861699(GAGE12I Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 861699(GAGE12I Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 861699(GAGE12I Antibody) at dilution 1/20. (Original magnification: ×200))
Product Categories/Family for anti-GAGE12I antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13kDa
NCBI Official Full Name
G antigen 12I
NCBI Official Synonym Full Names
G antigen 12I
NCBI Official Symbol
GAGE12I
NCBI Official Synonym Symbols
AL4; CT4.7; GAGE7B; GAGE-7B
NCBI Protein Information
G antigen 12I
UniProt Protein Name
G antigen 12I
Protein Family
UniProt Gene Name
GAGE12I
UniProt Synonym Gene Names
GAGE-12I
UniProt Entry Name
GG12I_HUMAN
NCBI Description
This gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The antigenic peptide YYWPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes. [provided by RefSeq, Jul 2008]
Research Articles on GAGE12I
1. The metazoan transcriptional regulator, Germ cell-less (GCL), as an interaction partner of GAGE12I.
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Product Notes
The GAGE12I gage12i (Catalog #AAA4758438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAGE12I Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GAGE12I can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the GAGE12I gage12i for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAGE12I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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