Rabbit anti-Human, Mouse SFRS2B Polyclonal Antibody | anti-SFRS2B antibody

SFRS2B, NT (SRSF8, SFRS2B, SRP46, Serine/arginine-rich splicing factor 8, Pre-mRNA-splicing factor SRP46, Splicing factor, arginine/serine-rich 2B) (FITC)

Cat. Num. AAA6346683

• Gene Names: SRSF8; DSM-1; SRP46; SFRS2B
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SFRS2B; Polyclonal Antibody; NT (SRSF8; SRP46; Serine/arginine-rich splicing factor 8; Pre-mRNA-splicing factor SRP46; Splicing factor; arginine/serine-rich 2B) (FITC); SRSF8; arginine/serine-rich 2B; anti-SFRS2B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SFRS2B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SFRS2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-73 amino acids from the N-terminal region of human SFRS2B.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SFRS2B Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SFRS2B antibody detected the SFRS2B protein (arrow).)

Western Blot (WB)

(SFRS2B Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the SFRS2B antibody detected the SFRS2B protein (arrow).)

Related Product Information for anti-SFRS2B antibody

The SR (serine/arginine-rich) family contains a number of phosphoproteins that function as essential and alternative splicing factors. The SR family of proteins is characterized by the presence of a ribonucleoprotein (RNP)-type RNA binding motif and a carboxyl-terminal arginine-serine-rich (RS) domain. The protein encoded by this gene is a member of the SR family and functions as an essential splicing factor in vitro. This gene is thought to be an expressed PR264/SC35 retropseudogene.

Product Categories/Family for anti-SFRS2B antibody

Antibodies; Splicing Factors

References

Manley, J.L., et al. Genes Dev. 24(11):1073-1074(2010). Lareau, L.F., et al. Nature 446(7138):926-929(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Li, J., et al. Mol. Cell. Biol. 23(21):7437-7447(2003). Grottke, C., et al. Int. J. Cancer 88(4):535-546(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 15055543
• NCBI GeneID: 10929
• NCBI Accession #: NP_115285.1
• NCBI GenBank Nucleotide #: NM_032102.3
• UniProt Accession #: Q9BRL6; Q6PF01; Q96TA3; B2R6B8
• Molecular Weight: 32,288 Da
• NCBI Official Full Name: serine/arginine-rich splicing factor 8
• NCBI Official Synonym Full Names: serine/arginine-rich splicing factor 8
• NCBI Official Symbol: SRSF8
• NCBI Official Synonym Symbols: DSM-1; SRP46; SFRS2B
• NCBI Protein Information: serine/arginine-rich splicing factor 8; SR splicing factor 8; splicing factor SRp46; pre-mRNA-splicing factor SRP46; splicing factor, arginine/serine-rich 2B; splicing factor, arginine/serine-rich, 46kD
• UniProt Protein Name: Serine/arginine-rich splicing factor 8
• UniProt Gene Name: SRSF8
• UniProt Synonym Gene Names: SFRS2B; SRP46; Splicing factor SRp46
• UniProt Entry Name: SRSF8_HUMAN

NCBI Description

This gene encodes a member of a family of proteins containing a ribonucleoprotein (RNP)-type RNA binding motif and a carboxyl-terminal arginine-serine-rich (RS) domain. The encoded protein functions as a pre-mRNA splicing factor. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

SRp46: Involved in pre-mRNA alternative splicing. Belongs to the splicing factor SR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 11q22

Cellular Component: nucleus

Molecular Function: protein binding; nucleotide binding

Biological Process: RNA splicing; mRNA processing