Rabbit SRSF5 Polyclonal Antibody | anti-SRSF5 antibody

SRSF5 Polyclonal Antibody

Cat. Num. AAA3007354

• Gene Names: SRSF5; HRS; SFRS5; SRP40
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SRSF5; Polyclonal Antibody; SRSF5 Polyclonal Antibody; Serine/arginine-rich splicing factor 5; Delayed-early protein HRS; Pre-mRNA-splicing factor SRP40; Splicing factor; arginine/serine-rich 5; HRS; SFRS5; SRP40; anti-SRSF5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SRSF5 polyclonal antibody detects endogenous levels of SRSF5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 272

• Applicable Applications for anti-SRSF5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 63-108 of Human SRSF5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SRSF5 polyclonal antibody at 1:500 dilutionLane1:RAW264. 7 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:NIH-3T3 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug))

Related Product Information for anti-SRSF5 antibody

Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns that are required for accurate splice site recognition and the control of alternative splicing. Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF. The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine (RS)-rich domain. They are not only essential for constitutive splicing but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites. An additional SR-associated protein, p32, tightly associates with SR factors and preferentially inhibits ASF/SF2 functioning as both a splicing enhancer and splicing repressor protein by preventing the stable interaction of ASF/SF2 and RNA.

NCBI and Uniprot Product Information

• NCBI GI #: 86991438
• NCBI GeneID: 6430
• NCBI Accession #: NP_001034554.1
• NCBI GenBank Nucleotide #: NP_001034554.1
• UniProt Accession #: Q13243
• Molecular Weight: ~ 91kDa
• NCBI Official Full Name: serine/arginine-rich splicing factor 5
• NCBI Official Synonym Full Names: serine and arginine rich splicing factor 5
• NCBI Official Symbol: SRSF5
• NCBI Official Synonym Symbols: HRS; SFRS5; SRP40
• NCBI Protein Information: serine/arginine-rich splicing factor 5
• UniProt Protein Name: Serine/arginine-rich splicing factor 5
• Protein Family: Serine/arginine-rich splicing factor
• UniProt Gene Name: SRSF5
• UniProt Synonym Gene Names: HRS; SFRS5; SRP40
• UniProt Entry Name: SRSF5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

SFRS5: Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Belongs to the splicing factor SR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA processing; Spliceosome

Chromosomal Location of Human Ortholog: 14q24

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nuclear speck; nucleus

Molecular Function: RS domain binding; protein binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA splice site selection; mRNA export from nucleus; regulation of cell cycle; RNA splicing; response to wounding; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription