Rabbit p63 Polyclonal Antibody | anti-CDC25B antibody

p63, phosphorylated (Ser160/162) (KET, p51, p40, AIS, p73L)

Cat. Num. AAA630152

• Reactivity: Chicken, Human, Mouse, Xenopus
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: p63; Polyclonal Antibody; phosphorylated (Ser160/162) (KET; p51; p40; AIS; p73L); Anti -p63; anti-CDC25B antibody

• Host: Rabbit
• Reactivity: Chicken, Human, Mouse, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of p63 only when phosphorylated at serine 160/162.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5,, 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-CDC25B antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser160/160 of human TAp63-a.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from untreated or nocodazole-treated (50 ng/ml, 24h) ME-180 cells, using Phospho-p63 (Ser160/162) Antibody (upper) or p63alpha P1001-34R (lower).)

Related Product Information for anti-CDC25B antibody

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription intiation site that yields a 40kD DNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the a, b and g isoforms (3,4). TAp63-a (fulllength) contains multiple serine residues followed by proline (Ser-Pro motif) that are potential cdk substrates expected to be phosphorylated in mitosis. Among these are Ser160, Ser162, Ser395, and Ser455.

Product Categories/Family for anti-CDC25B antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 243486
• NCBI GeneID: 994
• UniProt Accession #: O43550; P30305; Q6MZW8; Q7LDQ7
• Molecular Weight: 33,858 Da
• NCBI Official Full Name: p63
• NCBI Official Synonym Full Names: cell division cycle 25 homolog B (S. pombe)
• NCBI Official Symbol: CDC25B
• NCBI Protein Information: M-phase inducer phosphatase 2; OTTHUMP00000030137; OTTHUMP00000030138; OTTHUMP00000030139; cell division cycle 25B; dual specificity phosphatase Cdc25B
• UniProt Protein Name: Cdc25B phosphatase
• UniProt Gene Name: CDC25B
• UniProt Entry Name: O43550_HUMAN

NCBI Description

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq]

Uniprot Description

CDC25B: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.

Protein type: Nuclear receptor co-regulator; EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: positive regulation of cytokinesis; mitosis; cell division; positive regulation of protein kinase activity; positive regulation of cell proliferation; mitotic cell cycle; G2/M transition of mitotic cell cycle; positive regulation of mitotic cell cycle; oocyte maturation; protein amino acid phosphorylation; female meiosis I