Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tyrosine-protein kinase Srms (SRMS) Standard Curve.)
Mouse Tyrosine-Protein Kinase Srms (SRMS) ELISA Kit | SRMS elisa kit
Mouse Tyrosine-Protein Kinase Srms (SRMS) ELISA Kit
Gene Names
Srms; srm; A230069J08Rik
Reactivity
Mouse
Synonyms
Tyrosine-Protein Kinase Srms (SRMS); Mouse Tyrosine-Protein Kinase Srms (SRMS) ELISA Kit; SRMS; C20orf148; SRM; dJ697K14.1; SRMS elisa kit
Reactivity
Mouse
Specificity
Mouse Tyrosine-protein kinase Srms (SRMS) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SRMS. No significant cross-reactivity or interference between Mouse SRMS and analogues was observed.
Sequence Length
496
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tyrosine-protein kinase Srms (SRMS) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tyrosine-protein kinase Srms (SRMS) Standard Curve.)
Related Product Information for SRMS elisa kit
Description: This Mouse Tyrosine-protein kinase Srms (SRMS) ELISA Kit employs a two-site sandwich ELISA to quantitate SRMS in samples. An antibody specific for SRMS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SRMS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRMS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRMS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: It has SH2, SH2', and SH3 domains and a tyrosine residue for autophosphorylation in the kinase domain but lacks an N-terminal glycine for myristylation and a C-terminal tyrosine which, when phosphorylated, suppresses kinase activity. These are structural features of the recently identified Tec family of nonreceptor tyrosine kinases. The Srm N-terminal unique domain, however, lacks the structural characteristics of the Tec family kinases, and the sequence similarity is highest to Src in the SH region. Mutant mice were generated by gene targeting in embryonic stem cells but displayed no apparent phenotype as in mutant mice expressing Src family kinases. These results suggest that Srm constitutes a new family of nonreceptor tyrosine kinases that may be redundant in function.
Background: It has SH2, SH2', and SH3 domains and a tyrosine residue for autophosphorylation in the kinase domain but lacks an N-terminal glycine for myristylation and a C-terminal tyrosine which, when phosphorylated, suppresses kinase activity. These are structural features of the recently identified Tec family of nonreceptor tyrosine kinases. The Srm N-terminal unique domain, however, lacks the structural characteristics of the Tec family kinases, and the sequence similarity is highest to Src in the SH region. Mutant mice were generated by gene targeting in embryonic stem cells but displayed no apparent phenotype as in mutant mice expressing Src family kinases. These results suggest that Srm constitutes a new family of nonreceptor tyrosine kinases that may be redundant in function.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Tyrosine-protein kinase Srms
NCBI Official Synonym Full Names
src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites
NCBI Official Symbol
Srms
NCBI Official Synonym Symbols
srm; A230069J08Rik
NCBI Protein Information
tyrosine-protein kinase Srms
UniProt Protein Name
Tyrosine-protein kinase Srms
Protein Family
UniProt Gene Name
Srms
UniProt Synonym Gene Names
Srm
UniProt Entry Name
SRMS_MOUSE
Research Articles on SRMS
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Product Notes
The Mouse SRMS srms (Catalog #AAA9716867) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716867 ELISA Kit recognizes Mouse SRMS. It is sometimes possible for the material contained within the vial of "Tyrosine-Protein Kinase Srms (SRMS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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