Western Blot (WB)
(Western blot analysis of SRMS expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Rat SRMS Polyclonal Antibody | anti-SRMS antibody
SRMS Antibody
Gene Names
SRMS; SRM; PTK70; C20orf148; dJ697K14.1
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SRMS; Polyclonal Antibody; SRMS Antibody; C20orf148; dJ697K14.1; EC 2.7.1.112; Src related kinase lacking C terminal regulatory tyrosine and N terminal myristylation sites; SRM; SRMS_HUMAN; Tyrosine protein kinase Srms; Tyrosine-protein kinase Srms; anti-SRMS antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SRMS antibody detects endogenous levels of total SRMS
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
488
Applicable Applications for anti-SRMS antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Tissue Specificity
Highly expressed in most breast cancers (at protein level).
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of SRMS expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of SRMS expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9604196 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604196 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-SRMS antibody
Description: May be involved in proliferation or differentiation of keratinocytes in the skin.
Function: Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues (PubMed:23822091). Also phosphorylates KHDRBS1/SAM68 and VIM on tyrosine residues (PubMed:29496907). Phosphorylation of KHDRBS1 is EGF-dependent (PubMed:29496907).
Subunit Structure: Interacts (via the SH2 and SH3 domains) with DOK1 (PubMed:23822091). Interacts with KHDRBS1/SAM68 and VIM (PubMed:29496907).
Similarity: The N-terminal region regulates its kinase activity. Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Function: Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues (PubMed:23822091). Also phosphorylates KHDRBS1/SAM68 and VIM on tyrosine residues (PubMed:29496907). Phosphorylation of KHDRBS1 is EGF-dependent (PubMed:29496907).
Subunit Structure: Interacts (via the SH2 and SH3 domains) with DOK1 (PubMed:23822091). Interacts with KHDRBS1/SAM68 and VIM (PubMed:29496907).
Similarity: The N-terminal region regulates its kinase activity. Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 65 kDa
Predicted: 55 kDa
Predicted: 55 kDa
NCBI Official Full Name
tyrosine-protein kinase Srms
NCBI Official Synonym Full Names
src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites
NCBI Official Symbol
SRMS
NCBI Official Synonym Symbols
SRM; PTK70; C20orf148; dJ697K14.1
NCBI Protein Information
tyrosine-protein kinase Srms
UniProt Protein Name
Tyrosine-protein kinase Srms
Protein Family
UniProt Gene Name
SRMS
UniProt Synonym Gene Names
C20orf148
Uniprot Description
Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues (PubMed:23822091). Also phosphorylates KHDRBS1/SAM68 and VIM on tyrosine residues (PubMed:29496907). Phosphorylation of KHDRBS1 is EGF-dependent (PubMed:29496907).
Research Articles on SRMS
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Product Notes
The SRMS srms (Catalog #AAA9604196) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRMS Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRMS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the SRMS srms for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRMS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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