Goat SNAP-25 Polyclonal Antibody | anti-SNAP-25 antibody

GOAT ANTI SNAP-25 (C-TERMINAL)

Cat. Num. AAA221415

• Gene Names: SNAP25; RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: SNAP-25; Polyclonal Antibody; GOAT ANTI SNAP-25 (C-TERMINAL); anti-SNAP-25 antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 206

• Applicable Applications for anti-SNAP-25 antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 22kDa in human and mouse brain lysates. A minimum incubation time of 1 hour is recommended with this antibody.; ELISA: Maximum Dilution: 1/32000; Immunohistology - Paraffin: Minimum Dilution: 2.0; Maximum Dilution: 4.0ug/ml; Application Note: Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. MyBioSource recommend citrate buffer, pH6.0, for this purpose.; Western Blotting: Minimum Dilution: 0.01; Maximum Dilution: 0.03ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide; 0.5% Bovine Serum Albumin
• Buffer Solution: TRIS buffered saline
• Antiserum Preparation: Antisera to human SNAP-25 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-SNAP-25 antibody

Goat ati Human SNAP25 antibody recognizes human synaptosomal-associated protein, 25kD (SNAP-25), also known as Super protein (Sup) or Synaptosomal-associated 25 kDa protein. SNAP-25 is a 206 amino acid, presynaptic plasma membrane protein containning 2 http://www.ebi.ac.uk/interpro/entry/IPR000727 domains and is essential for Ca2+-regulated exocytosis of neurotransmitters. SNAP-25 binds to and negatively modulate Ca2+ channels, increasing the speed of neurotransmitter release (Rettiq et al. 1996).Goat ati Human SNAP25 antibody detects an epitope within the C-terminal (CT) of human SNAP25 SNAP-25 is expressed by neurons and neuroendocrine cells as two predominant isoforms; SNAP-25a and SNAP-25b. SNAP-25a is expressed early in development, whereas SNAP-25b expression is linked to synaptogenesis. Goat ati human SNAP25 antibody is expected to recognise both isoforms. SNAP-25 may be a possible therapeutic treatment target for sufferers of hyperactivity disorders (Barr et al. 2000), whilst SNAP-25 based peptides may be a possible treatment for involuntary muscle spasms Hodel 1998)..

NCBI and Uniprot Product Information

• NCBI GI #: 18765733
• NCBI GeneID: 6616
• NCBI Accession #: NP_003072.2
• NCBI GenBank Nucleotide #: NM_003081.3
• UniProt Accession #: P60880; P13795; P36974; P70557; P70558; Q53EM2; Q5U0B5; Q8IXK3; Q96FM2; B2RAU4; D3DW16; D3DW17
• Molecular Weight: 23,336 Da
• NCBI Official Full Name: synaptosomal-associated protein 25 isoform SNAP25A
• NCBI Official Synonym Full Names: synaptosomal-associated protein, 25kDa
• NCBI Official Symbol: SNAP25
• NCBI Official Synonym Symbols: RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
• NCBI Protein Information: synaptosomal-associated protein 25; SUP; resistance to inhibitors of cholinesterase 4 homolog; super protein; synaptosomal-associated protein, 25kD
• UniProt Protein Name: Synaptosomal-associated protein 25
• UniProt Gene Name: SNAP25
• UniProt Synonym Gene Names: SNAP; SNAP-25; SUP
• UniProt Entry Name: SNP25_HUMAN

NCBI Description

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 20p12-p11.2

Cellular Component: presynaptic membrane; SNARE complex; neuron projection; growth cone; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; trans-Golgi network; cell junction; cytosol; vesicle

Molecular Function: SNAP receptor activity; protein binding; syntaxin-1 binding; calcium-dependent protein binding

Biological Process: synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle exocytosis; glutamate secretion; neurotransmitter secretion; energy reserve metabolic process; neurotransmitter uptake; pathogenesis; synaptic vesicle priming; regulation of insulin secretion; synaptic vesicle docking during exocytosis

Disease: Myasthenic Syndrome, Congenital, 18