Mouse CD25 Monoclonal Antibody | anti-CD25 antibody

MOUSE ANTI DOG CD25

Cat. Num. AAA219878

• Gene Names: IL2RA; CD25; IL-2RA
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: CD25; Monoclonal Antibody; MOUSE ANTI DOG CD25; anti-CD25 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: P4A10
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 268

• Applicable Applications for anti-CD25 antibody: Flow cytometry (FC/FACS), Western Blot (WB)*
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.; Western Blot: Clone P4A10 detects a double band of approximately 55kDa in PMA-IO activated canine PBMC lysates. ; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Western Blotting: Application Note: Clone P4A10 produces a double band at approximately 55kDa under non-reducing conditions. A non-specific band may be observed at 116kDa
• Perservative Stabilisers: 0.09% Sodium Azide ; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Dog
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch

Testing Data

(Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25:FITC (MBS2130996F) and dual stained with Rat anti Dog CD4:Alexa Fluor647 )

Testing Data

(Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25:RPE (MBS2130996PE) and dual stained with Rat anti Dog CD4:Alexa Fluor647 )

Testing Data

(Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25 followed by Goat anti Mouse IgG:FITC (MBS235142) and dual stained with Rat anti Dog CD4:Alexa Fluor647 )

Related Product Information for anti-CD25 antibody

Mouse anti Dog CD25, clone P4A10 recognises the canine homologue of the human CD25 cell surface antigen, also known at IL-2Ralpha, a glycoprotein of approximately 55kDa expressed primarily by activated T lymphocytes (Abrams et al. 2010). The IL-2 receptor is composed of 3 subunits, an alpha chain (CD25), a beta chain (CD122) and a gamma chain (CD132), CD25 functions as a low affinity receptor for IL-2.Research has demonstrated that antibodies to CD4, FoxP3 and CD25 may be used in studies of T regulatory cells (Tregs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity. Previous studies of canine Tregs have utilized the mouse anti human CD25 monoclonal antibody, clone ACT1, which has demonstrated cross reactivity to canine CD25 (Rissetto et al. 2010). However this clone has poor affinity for canine CD25. The development of clone P4A10 now offers a specific monoclonal Mouse anti Canine CD25, demonstrating a greater affinity for canine CD25 than the cross reactive anti human antibody (Abrams et al. 2010).The dog is an important veterinary species in its own right. In addition dogs are used as an animal model in the study of a number of serious human disease states including various forms cancers (Paoloni et al. 2008), and in genetically related diseases of the hemopoitic system (Bauer et al. 2009). The generation of clone P4A10 provides an improved tool for the identification of canine CD25 and will facilitate studies in this species.

NCBI and Uniprot Product Information

• NCBI GI #: 50978976
• NCBI GeneID: 403870
• NCBI Accession #: NP_001003211.1
• NCBI GenBank Nucleotide #: NM_001003211.1
• UniProt Accession #: O62802
• Molecular Weight: 30,357 Da
• NCBI Official Full Name: interleukin-2 receptor subunit alpha
• NCBI Official Symbol: IL2RA
• NCBI Official Synonym Symbols: CD25; IL-2RA
• NCBI Protein Information: interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; interleukin 2 receptor alpha chain; interleukin-2 receptor alpha subunit; p55
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• UniProt Gene Name: IL2RA
• UniProt Synonym Gene Names: IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_CANFA

Uniprot Description

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.