Typical Testing Data/Standard Curve (for reference only)
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Human Synaptosomal-associated protein 25 ELISA Kit | SNAP25 elisa kit
Human Synaptosomal-associated protein 25 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 20p12-p11.2
Cellular Component: cell junction; cytoplasm; cytosol; growth cone; membrane; myelin sheath; neuron projection; perinuclear region of cytoplasm; plasma membrane; presynaptic membrane; SNARE complex; trans-Golgi network; vesicle; voltage-gated potassium channel complex
Molecular Function: calcium-dependent protein binding; protein binding; SNAP receptor activity; syntaxin-1 binding
Biological Process: associative learning; energy reserve metabolic process; glutamate secretion; locomotory behavior; neurotransmitter secretion; neurotransmitter uptake; regulation of insulin secretion; synaptic transmission; synaptic vesicle docking during exocytosis; synaptic vesicle exocytosis; synaptic vesicle fusion to presynaptic membrane; synaptic vesicle priming
Disease: Myasthenic Syndrome, Congenital, 18
Research Articles on SNAP25
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Product Notes
The Human SNAP25 snap25 (Catalog #AAA2883194) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2883194 ELISA Kit recognizes Human SNAP25. It is sometimes possible for the material contained within the vial of "Synaptosomal-associated protein 25, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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