Rabbit Catalase Polyclonal Antibody | anti-CAT antibody

Anti-Catalase Antibody

Cat. Num. AAA178425

• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Catalase; Polyclonal Antibody; Anti-Catalase Antibody; Cas1; CAT; Cs1; P04040; anti-CAT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 527

• Applicable Applications for anti-CAT antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Mouse, Rat; Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat; Tested Species:In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.
• Immunogen: E Coli-derived human Catalase recombinant protein (Position: E344-L527). Human Catalase shares 85.3% and 82.6% amino acid (aa) sequence identity with mouse and rat Catalase, respectively.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of Catalase expression in rat skeletal muscle extract (lane 1), mouse skeletal muscle extract (lane 2) and A549 whole cell lysates (lane 3). Catalase at 60KD was detected using rabbit anti- Catalase Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Immunohistochemistry (IHC)

(Catalase was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti- Catalase Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(Catalase was detected in paraffin-embedded sections of rat liver tissues using rabbit anti- Catalase Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(Catalase was detected in paraffin-embedded sections of human liver cancer tissues using rabbit anti- Catalase Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Related Product Information for anti-CAT antibody

Rabbit IgG polyclonal antibody for Catalase (CAT) detection.
Background: Catalase is a key antioxidant enzyme in the bodies defensing against oxidative stress. It is also a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene.

References

1. Junien, C., Turleau, C., de Grouchy, J., Said, R., Rethore, M.-O., Tenconi, R., Dufier, J. L. Regional assignment of catalase (CAT) gene to band 11p13: association with the aniridia-Wilms' tumor-gonadoblastoma (WAGR) complex. Ann. Genet. 23: 165-168, 1980.
2. Ogata, M., Wang, D.-H., Ogino, K. Mammalian acatalasemia: the perspectives of bioinformatics and genetic toxicology. Acta Med. Okayama 62: 345-361, 2008. Note: Erratum: Acta Med. Okayama 63: 121-122, 2009.
3. Quan, F., Korneluk, R. G., Tropak, M. B., Gravel, R. A. Isolation and characterization of the human catalase gene. Nucleic Acids Res. 14: 5321-5335, 1986.

NCBI and Uniprot Product Information

• NCBI GI #: 4557014
• NCBI GeneID: 847
• NCBI Accession #: NP_001743.1
• NCBI GenBank Nucleotide #: NM_001752.3
• UniProt Accession #: P04040; Q2M1U4; Q4VXX5; Q9BWT9; Q9UC85; A8K6C0; B2RCZ9; D3DR07
• Molecular Weight: 59,756 Da
• NCBI Official Full Name: catalase
• NCBI Official Synonym Full Names: catalase
• NCBI Official Symbol: CAT
• NCBI Protein Information: catalase
• UniProt Protein Name: Catalase
• Protein Family: Catalase
• UniProt Gene Name: CAT
• UniProt Entry Name: CATA_HUMAN

NCBI Description

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family.

Protein type: Endoplasmic reticulum; Energy Metabolism - methane; Apoptosis; Mitochondrial; Oxidoreductase; Amino Acid Metabolism - tryptophan; Hydrolase; EC 1.11.1.6

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: focal adhesion; intracellular membrane-bound organelle; membrane; mitochondrion; peroxisomal matrix; peroxisomal membrane; peroxisome

Molecular Function: antioxidant activity; catalase activity; enzyme binding; heme binding; NADP binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; receptor binding

Biological Process: hydrogen peroxide catabolic process; negative regulation of apoptosis; osteoblast differentiation; protein homotetramerization; protein tetramerization; purine nucleotide catabolic process; response to hydrogen peroxide; response to reactive oxygen species; UV protection

Disease: Acatalasemia