SNAP-25 Recombinant Protein | SNAP25 recombinant protein

SNAP-25 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120863

• Gene Names: SNAP25; RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: SNAP-25; SNAP-25 Protein; Human; Recombinant (His Tag); Human SNAP25/SUP Protein (His Tag); bA416N4.2 Protein; dJ1068F16.2 Protein; RIC-4 Protein; RIC4 Protein; SEC9 Protein; SNAP Protein; synaptosomal-associated protein; 25kDa; SNAP25 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly206

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human SNAP25 (P60880-1)(Met 1-Gly 206) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for SNAP25 recombinant protein

Background: Synaptosomal-associated protein 25, also known as Super protein, Synaptosomal-associated 25kDa protein, SNAP25 and SNAP, is a cytoplasm and cell membrane protein that belongs to the SNAP-25 family. SNAP25/SUP contains 2 t-SNARE coiled-coil homology domains. SNAP25/SUP is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25/SUP protein is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP25/SUP is a Q-SNARE protein contributing two alpha-helices in the formation of the exocytotic fusion complex in neurons where it assembles with syntaxin-1 and synaptobrevin. SNAP25/SUP is involved in the molecular regulation of neurotransmitter release. It may play an important role in the synaptic function of specific neuronal systems. SNAP25/SUP associates with proteins involved in vesicle docking and membrane fusion. SNAP25/SUP regulates plasma membrane recycling through its interaction with CENPF. SNAP25/SUP inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca²⁺-independent fashion. In glutamatergic synapses SNAP25/SUP decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.

References

Hodel A,et al.,1998, Int. J. Biochem. Cell Biol. 30 (10): 1069-73. Sudhof TC, et al.,2002, Nat Rev Neurosci 3 (8): 641-653. Chapman ER et al.,2002, Nat. Rev. Mol. Cell Biol. 3(7): 498-508. Chen X., et al., 2002, Neuron 33:397-409. Huang Q., et al., 2008, FEBS Lett. 582:1431-1436.

NCBI and Uniprot Product Information

• NCBI GI #: 18765733
• NCBI GeneID: 6616
• NCBI Accession #: NP_003072.2
• NCBI GenBank Nucleotide #: NM_003081.3
• UniProt Accession #: P60880; P13795; P36974; P70557; P70558; Q53EM2; Q5U0B5; Q8IXK3; Q96FM2; B2RAU4; D3DW16; D3DW17
• Molecular Weight: 23,315 Da
• NCBI Official Full Name: synaptosomal-associated protein 25 isoform SNAP25A
• NCBI Official Synonym Full Names: synaptosomal-associated protein, 25kDa
• NCBI Official Symbol: SNAP25
• NCBI Official Synonym Symbols: RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
• NCBI Protein Information: synaptosomal-associated protein 25; SUP; super protein; resistance to inhibitors of cholinesterase 4 homolog
• UniProt Protein Name: Synaptosomal-associated protein 25
• Protein Family: Synaptosomal-associated protein
• UniProt Gene Name: SNAP25
• UniProt Synonym Gene Names: SNAP; SNAP-25; SUP
• UniProt Entry Name: SNP25_HUMAN

NCBI Description

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 20p12-p11.2

Cellular Component: SNARE complex; presynaptic membrane; neuron projection; growth cone; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; trans-Golgi network; cell junction; cytosol; vesicle

Molecular Function: SNAP receptor activity; protein binding; syntaxin-1 binding; calcium-dependent protein binding

Biological Process: synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle exocytosis; glutamate secretion; neurotransmitter secretion; energy reserve metabolic process; neurotransmitter uptake; pathogenesis; synaptic vesicle priming; regulation of insulin secretion; synaptic vesicle docking during exocytosis

Disease: Myasthenic Syndrome, Congenital, 18