Immunofluorescence (IF)
(Immunofluorescence staining of SMEK1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SMEK1 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Rabbit anti-Human SMEK1 Polyclonal Antibody | anti-PPP4R3A antibody
Anti-SMEK1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
SMEK1; Polyclonal Antibody; Anti-SMEK1 Antibody; Rabbit Polyclonal; SMEK1/PPP4R3A Antibody; Rabbit PAb; Antigen Affinity Purified; SMEK homolog 1; suppressor of mek1 (Dictyostelium); Anti-FLFL1 Antibody; Anti-KIAA2010 Antibody; Anti-MSTP033 Antibody; Anti-PP4R3A Antibody; Anti-smk-1 Antibody; Anti-smk1 Antibody; anti-PPP4R3A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SMEK1/PPP4R3A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PPP4R3A antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:50-1:200
Immunogen
E Coli-derived Human SMEK1/PPP4R3A fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human SMEK1/PPP4R3A fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of SMEK1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SMEK1 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of SMEK1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SMEK1 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Related Product Information for anti-PPP4R3A antibody
PPP4R3A (Protein Phosphatase 4 Regulatory Subunit 3A) is a Protein Coding gene. The PPP4R3A gene, located on 14q32.12, is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, C.elegans, S.pombe, M.oryzae, N.crassa, A.thaliana, rice, and frog. PPP4R3A is widely expressed in bone marrow, testis, and other tissues. Among its related pathways are the Glucagon signaling pathway and Integrated Breast Cancer Pathway. PPP4R3A is a gene involved in Alzheimer's disease (AD) risk and progression. Given the protective effect of this variant, PPP4R3A should be further investigated as a gene of interest in neurodegenerative diseases and as a potential target for AD therapies. An important paralog of this gene is PPP4R3B.
References
Kim BR, et al. (2017) Bmi-1 interacts with smek1 and inactivates smek1-induced apoptotic cell death. Oncol Rep 37 (1): 579-586.Kim BR, et al. (2015) Smek1 inhibits endothelial cell proliferation by attenuating vegfr-2-dependent-akt/enos/hif-1alpha signaling pathways. Oncotarget 6 (31): 31830-31843.Christopher L, et al. (2017) A variant in ppp4r3a protects against alzheimer-related metabolic decline. Ann Neurol 82 (6): 900-911.
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Product Notes
The PPP4R3A (Catalog #AAA8109086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SMEK1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMEK1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:50-1:200. Researchers should empirically determine the suitability of the PPP4R3A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMEK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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