Western Blot (WB)
(Detection of Human/Mouse Smad2/3 by Western Blot.)
Goat anti-Human, Mouse Smad2,3 Polyclonal Antibody
Smad2,3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Smad2; 3; Polyclonal Antibody; 3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma); Anti -Smad2; anti-Smad2; 3 antibody
Host
Goat
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous human and mouse Smad3 on Western Blots. Reacts with recombinant human Smad2 (Accession # Q15796) and Smad3 in Western Blots. Predicted to detect rat
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.1ml is used, the concentration will be 1mg/ml.
Applicable Applications for anti-Smad2,3 antibody
Western Blot (WB)
Immunogen
E. coli derived recombinant human Smad3 (aa2-230)
Preparation and Storage
-20 degree C
Western Blot (WB)
(Detection of Human/Mouse Smad2/3 by Western Blot.)
Western Blot (WB)
(Detection of Human/Mouse Smad2/3 by Western Blot.)
Related Product Information for anti-Smad2,3 antibody
Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF- or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3.
Product Categories/Family for anti-Smad2,3 antibody
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Product Notes
The Smad2,3 (Catalog #AAA619908) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Smad2,3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Smad2,3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Smad2,3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad2,3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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