Chemiluminescence
(Representative blot from a previous lot. HepG2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Smad2/3 (1:1000 dilution). Proteins were visualized using a goat antirabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Smad2 (~55-60kD).)
Rabbit Smad2,3 Polyclonal Antibody
Smad2,3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Smad2; 3; Polyclonal Antibody; 3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma); Anti -Smad2; anti-Smad2; 3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Smad2 Mr 55-60kD and Smad3 Mr 50kD as determined by using Smad2 and Smad3 transfected Cos cells. Species Crossreactivity: Predicted to crossreact with mouse and rat based on conservation of immunogen sequence.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Applicable Applications for anti-Smad2,3 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000-1:2000 detects Smad2/3 in RIPA lysates of HepG2 cells. An additional unknown band was detected at Mr 97kD in the lysate.
Dilution: Western Blot: 1:1000-1:2000 detects Smad2/3 in RIPA lysates of HepG2 cells. An additional unknown band was detected at Mr 97kD in the lysate.
Immunogen
Fusion protein corresponding to amino acids 186-273 of human Smad2.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chemiluminescence
(Representative blot from a previous lot. HepG2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Smad2/3 (1:1000 dilution). Proteins were visualized using a goat antirabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Smad2 (~55-60kD).)
Chemiluminescence
(Representative blot from a previous lot. HepG2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Smad2/3 (1:1000 dilution). Proteins were visualized using a goat antirabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Smad2 (~55-60kD).)
Product Categories/Family for anti-Smad2,3 antibody
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Product Notes
The Smad2,3 (Catalog #AAA623276) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Smad2,3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Smad2,3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000-1:2000 detects Smad2/3 in RIPA lysates of HepG2 cells. An additional unknown band was detected at Mr 97kD in the lysate. Researchers should empirically determine the suitability of the Smad2,3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad2,3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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