Western Blot (WB)
(Western Blot analysis of SMAD8 in 15ug of A549 cell lysate using MBS615829 at 0.5ug/ml.)
Rabbit SMAD 1/5/8/9 Polyclonal Antibody
SMAD 1/5/8/9 (Small Mothers against Decapentaplegic Deleted in Pancreatic Carcinoma)
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G chromatography.
Synonyms
SMAD 1/5/8/9; Polyclonal Antibody; SMAD 1/5/8/9 (Small Mothers against Decapentaplegic Deleted in Pancreatic Carcinoma); Anti -SMAD 1/5/8/9 (Small Mothers against Decapentaplegic Deleted in Pancreatic Carcinoma); anti-SMAD 1/5/8/9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SMAD 1/5/8/9. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by Protein G chromatography.
Purified by Protein G chromatography.
Form/Format
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Applicable Applications for anti-SMAD 1/5/8/9 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 0.5-2ug/ml
Dilution: Western Blot: 0.5-2ug/ml
Immunogen
Synthetic peptides corresponding to amino acids 18-32, LGWKQGDEEEKWAEK, and 315-329, SNVNRNSTIENTRRH of human SMAD1. Species sequence homology: chicken, chimpanzee, invertebrates, mammals, porcine, Xenopus and zebrafish.
Positive Control
A549 whole cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of SMAD8 in 15ug of A549 cell lysate using MBS615829 at 0.5ug/ml.)
Western Blot (WB)
(Western Blot analysis of SMAD8 in 15ug of A549 cell lysate using MBS615829 at 0.5ug/ml.)
Related Product Information for anti-SMAD 1/5/8/9 antibody
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. For additional information on SMAD proteins, please follow the provided links to the NCBI website: human SMAD1, SMAD2, SMAD3, SMAD4, SMAD5, SMAD6, SMAD7, SMAD9 and mouse SMAD8.
Product Categories/Family for anti-SMAD 1/5/8/9 antibody
Similar Products
Product Notes
The SMAD 1/5/8/9 (Catalog #AAA615829) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMAD 1/5/8/9 (Small Mothers against Decapentaplegic Deleted in Pancreatic Carcinoma) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SMAD 1/5/8/9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 0.5-2ug/ml. Researchers should empirically determine the suitability of the SMAD 1/5/8/9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD 1/5/8/9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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