ECH1 recombinant protein

ECH1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121728

• Gene Names: ECH1; HPXEL
• Purity: >90% as determined by SDS-PAGE
• Synonyms: ECH1; ECH1 Protein; Human; Recombinant (His Tag); Human ECH1 Protein (His Tag); HPXEL Protein; enoyl CoA hydratase 1; peroxisomal; ECH1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 0.1M NaCl, 10% Glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Thr34-Leu328

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human ECH1 (Q13011)(Thr34-Leu328) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ECH1 recombinant protein

Background: ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

References

Kovalyov LI, et al. (2006) Polymorphism of delta3,5-delta2,4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue. Biochemistry Mosc. 71(4): 448-53. Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 127(3):635-48. FitzPatrick DR, et al. (1995) Isolation and characterization of rat and human cDNAs encoding a novel putative peroxisomal enoyl-CoA hydratase. Genomics. 27(3):457-66.

NCBI and Uniprot Product Information

• NCBI GI #: 70995211
• NCBI GeneID: 1891
• NCBI Accession #: NP_001389.2
• NCBI GenBank Nucleotide #: NM_001398.2
• UniProt Accession #: Q13011; Q8WVX0; Q96EZ9; A8K745
• Molecular Weight: 35,816 Da
• NCBI Official Full Name: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
• NCBI Official Synonym Full Names: enoyl CoA hydratase 1, peroxisomal
• NCBI Official Symbol: ECH1
• NCBI Official Synonym Symbols: HPXEL
• NCBI Protein Information: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta3,5-delta2,4-dienoyl-CoA isomerase; enoyl Coenzyme A hydratase 1, peroxisomal; peroxisomal enoyl-CoA hydratase 1
• UniProt Protein Name: Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
• UniProt Gene Name: ECH1
• UniProt Entry Name: ECH1_HUMAN

NCBI Description

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]

Uniprot Description

ECH1: Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4- trans-dienoyl-CoA. Belongs to the enoyl-CoA hydratase/isomerase family.

Protein type: Amino Acid Metabolism - tyrosine; Mitochondrial; Isomerase; EC 5.3.3.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: membrane; mitochondrion; peroxisome

Molecular Function: protein binding; isomerase activity; receptor binding

Biological Process: fatty acid beta-oxidation