Rabbit anti-Human, Mouse SLC9A9 Polyclonal Antibody | anti-SLC9A9 antibody

SLC9A9, CT (SLC9A9, NHE9, Sodium/hydrogen exchanger 9, Na(+)/H(+) exchanger 9, Solute carrier family 9 member 9) (APC)

Cat. Num. AAA6348584

• Gene Names: SLC9A9; NHE9; AUTS16
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SLC9A9; Polyclonal Antibody; CT (SLC9A9; NHE9; Sodium/hydrogen exchanger 9; Na(+)/H(+) exchanger 9; Solute carrier family 9 member 9) (APC); Solute carrier family 9 member 9; anti-SLC9A9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SLC9A9. Species Crossreactivity: Mouse. Cellular Localization: Late endosome membrane; multi-pass membrane protein
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SLC9A9 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 551-579 in the C-terminal of human SLC9A9 (NP_775924.1; Q8BZ00). UniProt Accession #Q8IVB4.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse spleen tissue lysates (35ug/lane) usingMBS648513. This demonstrates that MBS648513 detected SLC9A9 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using MBS6111726 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS648513 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells (right histogram) using MBS648513 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SLC9A9 antibody

This gene encodes a sodium hydrogen exchanger that is a member of the solute carrier 9 protein family. The encoded protein localizes the to the late recycling endosomes and may play an important role in maintaining cation homeostasis. Defects in this gene are associated with attention-deficit/hyperactivity disorder.

Product Categories/Family for anti-SLC9A9 antibody

Antibodies; Transport Proteins

References

1. Markunas, C.A., et al. Psychiatr. Genet. (2009). In press, 2. Vink, J.M., et al. Am. J. Hum. Genet. 84(3):367-379(2009), 3. Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8):1355-1358 (2008), 4. Ng, D., et al. Int. J. Cancer 123(7):1610-1615(2008), 5. Levy, D., et al. BMC Med. Genet. 8 SUPPL 1, S3 (2007), 5. Nakamura, N., et al. J. Biol. Chem. 280(2):1561-1572(2005). 6. de Silva, M.G., et al. J. Med. Genet. 40(10):733-740(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 27734935
• NCBI GeneID: 285195
• NCBI Accession #: NP_775924.1
• NCBI GenBank Nucleotide #: NM_173653.3
• UniProt Accession #: Q8IVB4; Q3LIC2; Q5JPI6; Q5WA58; Q8NAB9; A6NMQ9
• Molecular Weight: 72,565 Da
• NCBI Official Full Name: sodium/hydrogen exchanger 9
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE9, cation proton antiporter 9), member 9
• NCBI Official Symbol: SLC9A9
• NCBI Official Synonym Symbols: NHE9; AUTS16
• NCBI Protein Information: sodium/hydrogen exchanger 9; Na(+)/H(+) exchanger 9; sodium/proton exchanger NHE9; putative protein product of Nbla00118; solute carrier family 9 (sodium/hydrogen exchanger), isoform 9
• UniProt Protein Name: Sodium/hydrogen exchanger 9
• Protein Family: Sodium/hydrogen exchanger
• UniProt Gene Name: SLC9A9
• UniProt Synonym Gene Names: NHE9; NHE-9
• UniProt Entry Name: SL9A9_HUMAN

NCBI Description

This gene encodes a sodium/proton exchanger that is a member of the solute carrier 9 protein family. The encoded protein localizes the to the late recycling endosomes and may play an important role in maintaining cation homeostasis. Mutations in this gene are associated with autism susceptibility 16 and attention-deficit/hyperactivity disorder. [provided by RefSeq, Mar 2012]

Uniprot Description

NHE9: May act in electroneutral exchange of protons for Na(+) across membranes. Involved in the effusion of Golgi luminal H(+) in exchange for cytosolic cations. Involved in organelle ion homeostasis by contributing to the maintenance of the unique acidic pH values of the Golgi and post-Golgi compartments in the cell. A chromosomal aberration involving SLC9A9 has been found in a family with early-onset behavioral/developmental disorder with features of attention deficit-hyperactivity disorder and intellectual disability. Inversion inv(3)(p14:q21). The inversion disrupts DOCK3 and SLC9A9. Defects in SLC9A9 are a cause of susceptibility to autism type 16 (AUTS16). Autism is a complex multifactorial, pervasive developmental disorder characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Most individuals with autism also manifest moderate mental retardation. AUTS16 can be associated with epilepsy. Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.

Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: recycling endosome; late endosome membrane; integral to membrane

Molecular Function: protein binding; sodium:hydrogen antiporter activity

Biological Process: regulation of pH; ion transport; transmembrane transport

Disease: Autism, Susceptibility To, 16