Western Blot (WB)
(Western Blot analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.)
Rabbit Nectin 2 Polyclonal Antibody | anti-NECTIN2 antibody
Nectin 2 Polyclonal Antibody
Gene Names
NECTIN2; HVEB; PRR2; CD112; PVRL2; PVRR2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Nectin 2; Polyclonal Antibody; Nectin 2 Polyclonal Antibody; Herpesvirus entry mediator B; HveB; CD112; NECTIN2; HVEB; PRR2; PVRL2; CD 112; CD112 antigen; Herpes virus entry mediator B; Herpes virus entry protein B; Herpesvirus entry protein B; Hve B; Nectin-2; Nectin2; Poliovirus receptor like 2; Poliovirus receptor related 2 (herpesvirus entry mediator B); Poliovirus receptor related 2; Poliovirus receptor related protein 2; Poliovirus receptor-related protein 2; PRR 2; PVRL 2; PVRR 2; PVRR2; anti-NECTIN2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Nectin 2 polyclonal antibody detects endogenous levels of Nectin 2 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
538
Applicable Applications for anti-NECTIN2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
ICC: 1:50
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
ICC: 1:50
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein within human Nectin 2 aa 50-250.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.)
Western Blot (WB)
(Western Blot analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.)
Immunocytochemistry (ICC)
(ICC staining Nectin 2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Nectin 2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-NECTIN2 antibody
Nectin is a Ca2+-independent homophilic cell adhesion molecule that belongs to the immunoglobulin superfamily. Human nectin is identical to the poliovirus receptor-related protein (PRR) and has been identified as the alpha-herpesvirus entry mediator. Nectin constitutes a family consisting of at least Nectin 1, 2 and 3; each member has two or three splicing variants. Nectin 2, also designated PRR2/HveB, is ubquitously expressed, with the highest levels of expression in some human neuronal cell lines, fibroblastic cells, keratinocytes and primary activated T lymphocytes. Nectin 2 has two splicing variants, Nectin 2alpha (short form) and 2delta (long form). Both Nectin 2alpha and 2delta have a C-terminal conserved motif (E/A-X-Y-V). This motif interacts with the PDZ domain of the F-Actin-binding protein afadin, through which it is linked to the Actin cytoskeleton. The extracellular regions of the splicing variants are identical, but their transmembrane regions and cytoplasmic regions are unique. Nectin 2 mediates the entry of three mutant herpes simplex virus type 1 (HSV-1) strains that do not use HveA as co-receptor, but not wildtype HSV-1 strains. Nectin 2 also mediates the entry of HSV-2 and pseudorabies virus, but not bovine herpes virus type 1. Nectin 2delta is tyrosine phosphorylated in response to cell-cell adhesion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
58kDa
NCBI Official Full Name
Nectin-2
NCBI Official Synonym Full Names
nectin cell adhesion molecule 2
NCBI Official Symbol
NECTIN2
NCBI Official Synonym Symbols
HVEB; PRR2; CD112; PVRL2; PVRR2
NCBI Protein Information
nectin-2
UniProt Protein Name
Nectin-2
Protein Family
UniProt Gene Name
NECTIN2
UniProt Synonym Gene Names
Herpesvirus entry mediator B; HveB
NCBI Description
This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive (PubMed:26755705). Probable cell adhesion protein (PubMed:9657005).
Research Articles on NECTIN2
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Product Notes
The NECTIN2 nectin2 (Catalog #AAA3013107) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nectin 2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nectin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 ICC: 1:50 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the NECTIN2 nectin2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nectin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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