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Western Blot (WB) (Western Blot analysis in mouse spleen tissue lysates (35ug/lane) using MBS648513. This demonstrates that MBS648513 detected SLC9A9 protein (arrow).)

Rabbit anti-Human, Mouse SLC9A9 Polyclonal Antibody | anti-SLC9A9 antibody

SLC9A9, CT (SLC9A9, NHE9, Sodium/hydrogen exchanger 9, Na(+)/H(+) exchanger 9, Solute carrier family 9 member 9)

Gene Names
SLC9A9; NHE9; AUTS16
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
SLC9A9; Polyclonal Antibody; CT (SLC9A9; NHE9; Sodium/hydrogen exchanger 9; Na(+)/H(+) exchanger 9; Solute carrier family 9 member 9); Anti -SLC9A9; anti-SLC9A9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-SLC9A9 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
SLC9A9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 558-587 amino acids from the C-terminal region of human SLC9A9.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse spleen tissue lysates (35ug/lane) using MBS648513. This demonstrates that MBS648513 detected SLC9A9 protein (arrow).)

Western Blot (WB) (Western Blot analysis in mouse spleen tissue lysates (35ug/lane) using MBS648513. This demonstrates that MBS648513 detected SLC9A9 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using 047199 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS648513 for immunohistochemistry.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using 047199 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS648513 for immunohistochemistry.)

Testing Data

Testing Data
Related Product Information for anti-SLC9A9 antibody
This gene encodes a sodium hydrogen exchanger that is a member of the solute carrier 9 protein family. The encoded protein localizes the to the late recycling endosomes and may play an important role in maintaining cation homeostasis. Defects in this gene are associated with attention-deficit/hyperactivity disorder.
Product Categories/Family for anti-SLC9A9 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
72,565 Da
NCBI Official Full Name
sodium/hydrogen exchanger 9
NCBI Official Synonym Full Names
solute carrier family 9, subfamily A (NHE9, cation proton antiporter 9), member 9
NCBI Official Symbol
SLC9A9
NCBI Official Synonym Symbols
NHE9; AUTS16
NCBI Protein Information
sodium/hydrogen exchanger 9; Na(+)/H(+) exchanger 9; sodium/proton exchanger NHE9; putative protein product of Nbla00118; solute carrier family 9 (sodium/hydrogen exchanger), isoform 9
UniProt Protein Name
Sodium/hydrogen exchanger 9
Protein Family
UniProt Gene Name
SLC9A9
UniProt Synonym Gene Names
NHE9; NHE-9
UniProt Entry Name
SL9A9_HUMAN

NCBI Description

This gene encodes a sodium/proton exchanger that is a member of the solute carrier 9 protein family. The encoded protein localizes the to the late recycling endosomes and may play an important role in maintaining cation homeostasis. Mutations in this gene are associated with autism susceptibility 16 and attention-deficit/hyperactivity disorder. [provided by RefSeq, Mar 2012]

Uniprot Description

Function: May act in electroneutral exchange of protons for Na+ across membranes. Involved in the effusion of Golgi luminal H+ in exchange for cytosolic cations. Involved in organelle ion homeostasis by contributing to the maintenance of the unique acidic pH values of the Golgi and post-Golgi compartments in the cell. Ref.2

Subcellular location: Late endosome membrane; Multi-pass membrane protein Ref.2.

Tissue specificity: Ubiquitously expressed in all tissues tested. Expressed at highest levels in heart and skeletal muscle, followed by placenta, kidney, and liver. Expressed in the brain, in the medulla and spinal cord. Ref.1 Ref.2

Involvement in disease: A chromosomal aberration involving SLC9A9 has been found in a family with early-onset behavioral/developmental disorder with features of attention deficit-hyperactivity disorder and intellectual disability. Inversion inv3(p14:q21). The inversion disrupts DOCK3 and SLC9A9.Autism 16 (AUTS16) [MIM:613410]: A complex multifactorial, pervasive developmental disorder characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Most individuals with autism also manifest moderate mental retardation. AUTS16 can be associated with epilepsy.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.8

Sequence similarities: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. [View classification]

Sequence caution: The sequence BAC04005.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Research Articles on SLC9A9

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Product Notes

The SLC9A9 slc9a9 (Catalog #AAA648513) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC9A9, CT (SLC9A9, NHE9, Sodium/hydrogen exchanger 9, Na(+)/H(+) exchanger 9, Solute carrier family 9 member 9) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SLC9A9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the SLC9A9 slc9a9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC9A9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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