Western Blot (WB)
(Western blot analysis of SHISA4 in human brain tissue lysate with SHISA4 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit anti-Human, Mouse SHISA4 Polyclonal Antibody | anti-SHISA4 antibody
SHISA4 Antibody
Gene Names
SHISA4; TMEM58; C1orf40
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
SHISA4 Antibody is affinity chromatography purified via peptide column.
Synonyms
SHISA4; Polyclonal Antibody; SHISA4 Antibody; TMEM58; C1orf40; UNQ583/PRO1153; Protein shisa-4; Transmembrane protein 58; shisa homolog 4 (Xenopus laevis); anti-SHISA4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SHISA4 antibody is predicted to not cross-react with other SHISA protein family members.
Purity/Purification
SHISA4 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
197
Applicable Applications for anti-SHISA4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
SHISA4 Antibody can be used for detection of SHISA4 by Western blot starting at 1 mug/mL.
Conjugate
Unconjugated
Immunogen
SHISA4 antibody was raised against a 15 amino acid peptide near the center of human SHISA4.
Buffer
SHISA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SHISA4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of SHISA4 in human brain tissue lysate with SHISA4 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of SHISA4 in human brain tissue lysate with SHISA4 antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SHISA4 in mouse brain tissue with SHISA4 antibody at 2.5 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SHISA4 in mouse brain tissue with SHISA4 antibody at 2.5 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of SHISA4 in mouse brain tissue with SHISA4 antibody at 20 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of SHISA4 in mouse brain tissue with SHISA4 antibody at 20 μg/ml.)
Related Product Information for anti-SHISA4 antibody
SHISA4 Antibody: SHISA4 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 22 kDa
Observed: 24 kDa
Observed: 24 kDa
NCBI Official Full Name
protein shisa-4
NCBI Official Synonym Full Names
shisa family member 4
NCBI Official Symbol
SHISA4
NCBI Official Synonym Symbols
TMEM58; C1orf40
NCBI Protein Information
protein shisa-4; shisa homolog 4; transmembrane protein 58
UniProt Protein Name
Protein shisa-4
Protein Family
UniProt Gene Name
SHISA4
UniProt Synonym Gene Names
C1orf40; TMEM58
UniProt Entry Name
SHSA4_HUMAN
Uniprot Description
SHISA4: Belongs to the shisa family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: integral to membrane
Similar Products
Product Notes
The SHISA4 shisa4 (Catalog #AAA153348) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHISA4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SHISA4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). SHISA4 Antibody can be used for detection of SHISA4 by Western blot starting at 1 mug/mL. Researchers should empirically determine the suitability of the SHISA4 shisa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHISA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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