Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Agrin (AGRN) Standard Curve.)
Mouse Agrin (AGRN) ELISA Kit | AGRN elisa kit
Mouse Agrin (AGRN) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Agrin (AGRN) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Agrin (AGRN) Standard Curve.)
Background: Agrin is a large proteoglycan whose best characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. This protein has nine domains homologous to protease inhibitors. It may also have functions in other tissues and during other stages of development. It is major proteoglycan component in glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.This protein binds to several receptors on the surface of skeletal muscle. The receptor which seems to be required for formation of the neuromuscular junction (NMJ) is called the MuSK receptor (Muscle specific kinase).
NCBI and Uniprot Product Information
Uniprot Description
AGRN: Plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Ligand of the MUSK signaling complex that directly binds LRP4 in this complex and induces the phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Defects in AGRN are a cause of myasthenia, limb-girdle, familial (LGM). A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.
Protein type: Cell development/differentiation; Extracellular matrix; Secreted; Secreted, signal peptide; Cell adhesion
Cellular Component: basal lamina; basement membrane; cell junction; cell surface; cytoplasm; extracellular matrix; extracellular space; integral to membrane; membrane; plasma membrane; sarcolemma; synapse
Molecular Function: acetylcholine receptor regulator activity; ATPase inhibitor activity; calcium ion binding; heparan sulfate proteoglycan binding; protein binding; transforming growth factor beta binding
Biological Process: cell differentiation; multicellular organismal development; neuromuscular junction development; neurotransmitter receptor metabolic process; plasma membrane organization and biogenesis; positive regulation of filopodium formation; positive regulation of GTPase activity; positive regulation of neuron apoptosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of synaptic growth at neuromuscular junction; positive regulation of transcription from RNA polymerase II promoter; protein heterotetramerization; receptor clustering; regulation of GTPase activity; regulation of protein amino acid phosphorylation; regulation of synaptic growth at neuromuscular junction; synaptic transmission; synaptogenesis
Research Articles on AGRN
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Product Notes
The Mouse AGRN agrn (Catalog #AAA9717179) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717179 ELISA Kit recognizes Mouse AGRN. It is sometimes possible for the material contained within the vial of "Agrin (AGRN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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