Rabbit RSPO1 Polyclonal Antibody | anti-RSPO1 antibody

RSPO1 Antibody

Cat. Num. AAA150333

• Gene Names: RSPO1; RSPO; CRISTIN3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: RSPO1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: RSPO1; Polyclonal Antibody; RSPO1 Antibody; RSPO; CRISTIN3; R-spondin-1; Roof plate-specific spondin-1; hRspo1; R-spondin homolog (Xenopus laevis); anti-RSPO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: RSPO1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 263

• Applicable Applications for anti-RSPO1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: RSPO1 antibody can be used for detection of RSPO1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: RSPO1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human RSPO1.
• Buffer: RSPO1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: RSPO1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RSPO1 in human heart tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RSPO1 in human heart tissue with RSPO1 antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of RSPO1 in Human Spleen tissue with RSPO1 antibody at 20 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 2.5 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.Green: RSPO1 antibody (5171)Red: Phylloidin stainingBlue: DAPI staining)

Related Product Information for anti-RSPO1 antibody

RSPO1 Antibody: RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels. At least two isoforms of RSPO1 are known to exist. RSPO1 antibody will not cross-react with RSPO family members.

NCBI and Uniprot Product Information

• NCBI GI #: 84490388
• NCBI GeneID: 284654
• NCBI Accession #: NP_001033722
• NCBI GenBank Nucleotide #: NM_001038633.3
• UniProt Accession #: Q2MKA7; Q0H8S6; Q14C72; Q5T0F2; Q8N7L5; A2A420
• Molecular Weight: 21,775 Da
• NCBI Official Full Name: R-spondin-1 isoform 1
• NCBI Official Synonym Full Names: R-spondin 1
• NCBI Official Symbol: RSPO1
• NCBI Official Synonym Symbols: RSPO; CRISTIN3
• NCBI Protein Information: R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
• UniProt Protein Name: R-spondin-1
• Protein Family: R-spondin
• UniProt Gene Name: RSPO1
• UniProt Synonym Gene Names: hRspo1
• UniProt Entry Name: RSPO1_HUMAN

NCBI Description

This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cell adhesion; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: extracellular space; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding

Biological Process: regulation of gene expression; regulation of receptor internalization; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation; male meiosis; positive regulation of Wnt receptor signaling pathway

Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal