Western Blot (WB)
(WB analysis of HEK293 cell llysate. PVDF membrane was probed with MBS6010231 (1ug/ml) and HRP-conjugated anti-sheep secondary antibody.)
Sheep anti-Human SHISA4 Polyclonal Antibody | anti-SHISA4 antibody
SHISA4 (C1orf40, PRMP, TMEM58, Protein shisa-4 homolog)
Gene Names
SHISA4; TMEM58; C1orf40
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
SHISA4; Polyclonal Antibody; SHISA4 (C1orf40; PRMP; TMEM58; Protein shisa-4 homolog); Anti -SHISA4 (C1orf40; anti-SHISA4 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human and mouse Shisa-4 extracellular domain.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-SHISA4 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Direct ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Dilution: Direct ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunogen
Recombinant corresponding to human Shisa-4 extracellular domain expressed in E. coli (Q96DD7).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(WB analysis of HEK293 cell llysate. PVDF membrane was probed with MBS6010231 (1ug/ml) and HRP-conjugated anti-sheep secondary antibody.)
Western Blot (WB)
(WB analysis of HEK293 cell llysate. PVDF membrane was probed with MBS6010231 (1ug/ml) and HRP-conjugated anti-sheep secondary antibody.)
Related Product Information for anti-SHISA4 antibody
Shisa-4 (Shisa is a form of Japanese sculpture that involves faces/heads; also known as C1orf40, PRMP and TMEM58) is a 26-32kD member of the Shisa-4 subfamily, Shisa family of proteins. It may be found associated with the ER of embryonic limb bud cells, and based on other Shisa proteins, perhaps antagonizes either Wnt or FGF signaling. Shisa-4 is also described as a plasma membrane protein on oligodendroglia that links the ECM to the actin cytoskeleton. Mature human Shisa-4 is a 170 (aa) type I transmembrane protein. It contains a 60aa extracellular domain (aa28-87) plus an 89aa cytoplasmic region that possesses a Pro-rich segment (aa131-195). Over (aa29-85), human Shisa-4 shares 96% aa identity with mouse Shisa-4.
Product Categories/Family for anti-SHISA4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,522 Da
NCBI Official Full Name
SHISA4 protein
NCBI Official Synonym Full Names
shisa family member 4
NCBI Official Symbol
SHISA4
NCBI Official Synonym Symbols
TMEM58; C1orf40
NCBI Protein Information
protein shisa-4; shisa homolog 4; transmembrane protein 58
UniProt Protein Name
Protein shisa-4
Protein Family
UniProt Gene Name
SHISA4
UniProt Synonym Gene Names
C1orf40; TMEM58
UniProt Entry Name
SHSA4_HUMAN
Uniprot Description
SHISA4: Belongs to the shisa family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: integral to membrane
Similar Products
Product Notes
The SHISA4 shisa4 (Catalog #AAA6010231) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The SHISA4 (C1orf40, PRMP, TMEM58, Protein shisa-4 homolog) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SHISA4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Direct ELISA: 0.5-1ug/ml Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the SHISA4 shisa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHISA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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