Testing Data
Rabbit VASA/DDX4 Polyclonal Antibody | anti-VASA/DDX4 antibody
Rabbit anti VASA/DDX4
Reactivity
Human, rat, mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The rabbit IgG is purified by Epitope Affinity Purification.
Synonyms
VASA/DDX4; Polyclonal Antibody; Rabbit anti VASA/DDX4; Rabbit anti VASA/DDX4 Polyclonal antibody; VASA Protein; DEAD-box protein 4 (DDX4); Mab4611; anti-VASA/DDX4 antibody
Host
Rabbit
Reactivity
Human, rat, mouse
Clonality
Polyclonal
Specificity
This antibody recognizes ~76 kDa of VASA protein. This antibody also reacts with human, mouse and rat. The other species are not tested.
Purity/Purification
The rabbit IgG is purified by Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile phosphatebuffered saline (pH7.2) containing antibody stabilizer.
Concentration
100 ug/200 ul (varies by lot)
Applicable Applications for anti-VASA/DDX4 antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Suggested Dilution:
Western Blot: 0.1-1 ug/m
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/m
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Antigen Preparation
A synthetic peptide corresponding to the N-terminus of human VASA protein. This sequence is identical within human, rat, mouse.
Positive Control
Mouse embryonic germ line cell
Cellular Localization
Cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C to 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles
Testing Data
Testing Data
Related Product Information for anti-VASA/DDX4 antibody
The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for gemr cell formation. VASA is therefore a highly specific marker of germ cells. This protein is located in cytoplasmic and is present only in migratory primordial germ cells in the region of the gonadal ridge. On testicular sections, VASA expression is the highest in spermatogonia, reduced in spermatocytes, low in spermatids and absent in sperm. In the ovary, VASA expression the highest in oogonia but persists throughout oogenesis. VASA has a glycine-rich N-terminus with multiple repeats of an RGG motif believed to function in RNA binding. The phosphorylation of VASA at N-terminus may regulate the translation of intricate mRNAs that are essential for differentiation.
References
• Matthew Wawersik, et al. Somatic control of germline sexual development is mediated by the JAK/STAT pathway. Nature. 2005 July 28; 436(7050): 563-567.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The VASA/DDX4 (Catalog #AAA462150) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti VASA/DDX4 reacts with Human, rat, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VASA/DDX4 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Suggested Dilution: Western Blot: 0.1-1 ug/m ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the VASA/DDX4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VASA/DDX4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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