Testing Data
Rabbit MMP-13 Polyclonal Antibody | anti-MMP-13 antibody
Rabbit anti MMP-13
Reactivity
Human, rat, mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The rabbit IgG is purified by Epitope Affinity Purification.
Synonyms
MMP-13; Polyclonal Antibody; Rabbit anti MMP-13; Rabbit anti MMP13 antibody; Matrix Metalloproteinase-13; Collagenase-3; anti-MMP-13 antibody
Host
Rabbit
Reactivity
Human, rat, mouse
Clonality
Polyclonal
Specificity
This antibody recognizes ~60 and 48 kDa human MMP- 13. It also reacts with mouse and rat. The other species are not tested
Purity/Purification
The rabbit IgG is purified by Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
100 ug/200 ul (varies by lot)
Applicable Applications for anti-MMP-13 antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Suggested Dilution:
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
Antigen Preparation
A synthetic peptide corresponding to the hinge of human MMP13 protein. This sequence is identical among the human, mouse and rat origins.
Positive Control
SW1353 cell
Cellular Localization
Cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C to 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-MMP-13 antibody
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Unpaired specific MMPs by tissue inhibitor of MMPs (TIMPs) are related in the invasive tumor processes. Interstitial collagenase (MMP-1)(52 kDa), gelatinase A/type IV collagenase (MMP-2) (72 kDa), and gelatinase B/type V collagenase (MMP-9) (92 kDa) dissolve extracellular matrix (ECM) and may initiate and promote angiogenesis. Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. This protein is secreted as a 60 kDa proenzyme and active form of 48 kDa by cleavage.
References
• Tania R. Lizarbe, et al. Nitric oxide elicits functional MMP-13 protein-tyrosine nitration during wound repair. The FASEB Journal. 2008;22:3207-3215.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The MMP-13 (Catalog #AAA462080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti MMP-13 reacts with Human, rat, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MMP-13 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Suggested Dilution: Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 1:100. Researchers should empirically determine the suitability of the MMP-13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP-13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
