Western Blot (WB)
(Western blot analysis of recombinant RFP (lane 1 & 2), recombinant YFP (lane 3 & 4) and recombinant EGFP (lane 5 & 6) probed with Rabbit anti GFP antibody)
Rabbit Green Fluorescent Protein Polyclonal Antibody | anti-GFP antibody
Rabbit anti Green Fluorescent Protein
Applications
Western Blot
Purity
Purified whole serum by affinity chromatography.
Synonyms
Green Fluorescent Protein; Polyclonal Antibody; Rabbit anti Green Fluorescent Protein; Green Fluorescent Protein Antibody; anti-GFP antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified whole serum by affinity chromatography.
Form/Format
Liquid in phosphate buffered saline with 30% glycerol, 0.5% bovine serum albumin, 0.01% thiomersal.
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
238
Applicable Applications for anti-GFP antibody
Western Blot (WB)
Application Notes
WB: 0.5-4.0 ug/ml
Immunogen
E Coli expressed recombinant EGFP
Preperation
Antiserum to green fluorescent protein (GFP) was raised by repeated immunisation of rabbits with highly purified antigen.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Western Blot (WB)
(Western blot analysis of recombinant RFP (lane 1 & 2), recombinant YFP (lane 3 & 4) and recombinant EGFP (lane 5 & 6) probed with Rabbit anti GFP antibody)
Western Blot (WB)
(Western blot analysis of recombinant RFP (lane 1 & 2), recombinant YFP (lane 3 & 4) and recombinant EGFP (lane 5 & 6) probed with Rabbit anti GFP antibody)
Related Product Information for anti-GFP antibody
Rabbit anti GFP antibody recognizes green fluorescent protein (GFP) and its variants. GFP is a ~27 kDa protein derived from the jellyfish Aequorea victoria. GFP fluoresces green (509 nm) when excited by blue light (395 nm), and is commonly used as a marker of gene expression. Rabbit anti GFP antibody is known to cross-react with enhanced green fluorescent protein (EGFP), yellow fluorescent protein (YFP) and red fluorescent protein (RFP).
NCBI and Uniprot Product Information
Uniprot Description
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.
Similar Products
Product Notes
The Green Fluorescent Protein gfp (Catalog #AAA225345) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Green Fluorescent Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.5-4.0 ug/ml. Researchers should empirically determine the suitability of the Green Fluorescent Protein gfp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Green Fluorescent Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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