Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SEC16B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Rabbit anti-Human, Mouse SEC16B Polyclonal Antibody | anti-SEC16B antibody
SEC16B Polyclonal Antibody
Gene Names
SEC16B; RGPR; LZTR2; SEC16S; PGPR-p117; RGPR-p117
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
SEC16B; Polyclonal Antibody; SEC16B Polyclonal Antibody; LZTR2; PGPR-p117; RGPR; SEC16S; anti-SEC16B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
DPPASAAWSPVQADVSSAGPKAPMKFYIPHVPVSFGPGGQLVHVGPSSPTDGQAALVELHSMEVILNDSEEQEEMRSFSGPLIREDVHKVDIMTFCQQKAAQSCKSETLGSRDSALLWQLLVLLCRQNGSMVGSDIAELLMQDCKKLEKYKRQPPVANLINLTDEDWPVLSSGTPNLLTGEIPPSVETPAQIVEKFTRLLYYGRKKEALEWAMKNHLWGHALFLSSKMDPQTYSWVMSGFTSTLALNDPLQTLFQ
Sequence Length
1060
Applicable Applications for anti-SEC16B antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human SEC16B
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Endoplasmic reticulum membrane, Golgi apparatus membrane, Peripheral membrane protein
Positive Samples
HeLa, Mouse pancreas
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SEC16B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SEC16B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Product Categories/Family for anti-SEC16B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 59kDa; 70kDa; 116kDa
Observed: 117kDa
Observed: 117kDa
NCBI Official Full Name
protein transport protein Sec16B isoform 1
NCBI Official Synonym Full Names
SEC16 homolog B, endoplasmic reticulum export factor
NCBI Official Symbol
SEC16B
NCBI Official Synonym Symbols
RGPR; LZTR2; SEC16S; PGPR-p117; RGPR-p117
NCBI Protein Information
protein transport protein Sec16B
UniProt Protein Name
Protein transport protein Sec16B
Protein Family
UniProt Gene Name
SEC16B
UniProt Synonym Gene Names
KIAA1928; LZTR2; RGPR; SEC16S; RGPR-p117
NCBI Description
SEC16B is a mammalian homolog of S. cerevisiae Sec16 that is required for organization of transitional endoplasmic reticulum (ER) sites and protein export (Bhattacharyya and Glick, 2007 [PubMed 17192411]).[supplied by OMIM, Jun 2009]
Uniprot Description
Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus and for normal transitional endoplasmic reticulum (tER) organization.
Research Articles on SEC16B
1. Significant associations were identified at 3.5 years for SEC16B rs10913469 with dietary parameters
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Product Notes
The SEC16B sec16b (Catalog #AAA9134672) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEC16B Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SEC16B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the SEC16B sec16b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DPPASAAWSP VQADVSSAGP KAPMKFYIPH VPVSFGPGGQ LVHVGPSSPT DGQAALVELH SMEVILNDSE EQEEMRSFSG PLIREDVHKV DIMTFCQQKA AQSCKSETLG SRDSALLWQL LVLLCRQNGS MVGSDIAELL MQDCKKLEKY KRQPPVANLI NLTDEDWPVL SSGTPNLLTG EIPPSVETPA QIVEKFTRLL YYGRKKEALE WAMKNHLWGH ALFLSSKMDP QTYSWVMSGF TSTLALNDPL QTLFQ. It is sometimes possible for the material contained within the vial of "SEC16B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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