Rabbit anti-Human, Mouse CYP2R1 Polyclonal Antibody | anti-CYP2R1 antibody

CYP2R1, ID (Vitamin D 25-hydroxylase, Cytochrome P450 2R1)

Cat. Num. AAA626218

• Gene Names: CYP2R1; MGC4663
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: CYP2R1; Polyclonal Antibody; ID (Vitamin D 25-hydroxylase; Cytochrome P450 2R1); Anti -CYP2R1; anti-CYP2R1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP2R1. Species Crossreactivity: mouse.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CYP2R1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:10-1:50
• Immunogen: Synthetic peptide selected from the Center region of human CYP2R1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CYP2R1 Antibody (Center) in NCI-H460 cell line(lane 1) and mouse liver tissue(lane 2) lysates (35ug/lane). CYP2R1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with CYP2R1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-CYP2R1 antibody

CYP2R1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in CYP2R1 gene has been associated with selective 25-hydroxyvitamin D deficiency.

Product Categories/Family for anti-CYP2R1 antibody

Antibodies; Abs to Enzymes; Cytochrome

NCBI and Uniprot Product Information

• NCBI GI #: 119588879
• NCBI GeneID: 120227
• UniProt Accession #: Q6VVX0; Q2M3H3; Q5RT65
• Molecular Weight: 57,359 Da
• NCBI Official Full Name: cytochrome P450, family 2, subfamily R, polypeptide 1, isoform CRA_d
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily R, polypeptide 1
• NCBI Official Symbol: CYP2R1
• NCBI Official Synonym Symbols: MGC4663
• NCBI Protein Information: vitamin D 25-hydroxylase; OTTHUMP00000231476; cytochrome P450 2R1; cytochrome P450, family 2, R1
• UniProt Protein Name: Vitamin D 25-hydroxylase
• Protein Family: Vitamin D 25-hydroxylase
• UniProt Gene Name: CYP2R1
• UniProt Entry Name: CP2R1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in this gene has been associated with selective 25-hydroxyvitamin D deficiency. [provided by RefSeq]

Uniprot Description

CYP2R1: Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3). Defects in CYP2R1 are the cause of rickets vitamin D- dependent type 1B (VDDR1B); also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activityand low levels of 25-hydroxyvitamin D. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; EC 1.14.13.15

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; steroid hydroxylase activity; oxygen binding; vitamin D3 25-hydroxylase activity

Biological Process: organic acid metabolic process; steroid metabolic process; vitamin metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; vitamin D metabolic process

Disease: Vitamin D Hydroxylation-deficient Rickets, Type 1b