Rat CD8 ALPHA Monoclonal Antibody | anti-CD8 ALPHA antibody

RAT ANTI MOUSE CD8 ALPHA:APC

Cat. Num. AAA210027

• Gene Names: Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD8 ALPHA; Monoclonal Antibody; RAT ANTI MOUSE CD8 ALPHA:APC; anti-CD8 ALPHA antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: KT15
• Form/Format: APC; Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
• Sequence Length: 247

• Applicable Applications for anti-CD8 ALPHA antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 1 ml distilled water; Perservative Stabilisers
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data

(Staining of mouse spleen cells with Rat anti Mouse CD8 (MBS213104))

Testing Data

(Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG (MBS235195) s a detection reagent. Low power)

Testing Data

(Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE (MBS213860))

Testing Data

(Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha:RPE (MBS213859))

Testing Data

(Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE - Alexa Fluor 647 )

Testing Data

(Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha: Biotin (MBS210507))

Testing Data

(Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG (MBS235195) s a detection reagent. Medium power)

Testing Data

(Staining of mouse spleen with Rat anti Mouse CD8 Alpha: FITC (MBS211064))

Testing Data

(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD8 Alpha: Alexa Fluor 488 )

Testing Data

(Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG (MBS235195) s a detection reagent. High power)

NCBI and Uniprot Product Information

• NCBI GI #: 124487437
• NCBI GeneID: 12525
• NCBI Accession #: NP_001074579.1
• NCBI GenBank Nucleotide #: NM_001081110.2
• Molecular Weight: 27,456 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8 antigen, alpha chain
• NCBI Official Symbol: Cd8a
• NCBI Official Synonym Symbols: Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein Lyt-2
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• UniProt Gene Name: Cd8a
• UniProt Synonym Gene Names: Lyt-2; Lyt2
• UniProt Entry Name: CD8A_MOUSE

Uniprot Description

CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; protein kinase binding

Biological Process: cell surface receptor linked signal transduction; T cell activation; immune system process; T cell mediated immunity; positive regulation of calcium-mediated signaling; cytotoxic T cell differentiation; defense response to virus