Chemiluminescence
((0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SAP97 Polyclonal Antibody
SAP97 (Disks Large Homolog 1, Synapse-associated Protein 97, SAP-97, DLG1, hDlg)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
SAP97; Polyclonal Antibody; SAP97 (Disks Large Homolog 1; Synapse-associated Protein 97; SAP-97; DLG1; hDlg); Anti -SAP97 (Disks Large Homolog 1; anti-SAP97 antibody
Host
Goat
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes human DLG1. Species sequence homology: Mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Applicable Applications for anti-SAP97 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:16,000
Western Blot: 0.3-1ug/ml, observed in lysates of cell line KELLY on ~150kD bands
Dilution: ELISA: 1:16,000
Western Blot: 0.3-1ug/ml, observed in lysates of cell line KELLY on ~150kD bands
Immunogen
Synthetic peptide corresponding to C-NSKTRDKGEIPDD, from human DLG1, at the internal region of the protein (NP_001091894.1, NP_004078.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chemiluminescence
((0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
((0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-SAP97 antibody
Synapse-associated protein (SAP) 97 is a 97kD membrane-associated synapse protein with extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal. SAP97 consists of three 90aa PDZ domains at its amino terminus, followed by a Src homology (SH) 3 domain, and a region with homology to guanylate kinases (GK). In addition to the CNS, SAP97 is also detected at the basal lateral membrane between a variety of epithelial cells. SAP97 is recruited to the submembranous cortical cytoskeleton at cell-cell contact sites as part of the E-cadherin complex. Co-expression of SAP97 and K+ channel results in the colocalization of these proteins in large round intracellular aggregates. SAP 97 has been shown to have functional homology to Drosophila tumor suppressor protein (DlgA), a PSD 95 related protein involved in maintaining integrity of epithelial and neuromuscular junctions.
Product Categories/Family for anti-SAP97 antibody
Similar Products
Product Notes
The SAP97 (Catalog #AAA6011539) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The SAP97 (Disks Large Homolog 1, Synapse-associated Protein 97, SAP-97, DLG1, hDlg) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SAP97 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:16,000 Western Blot: 0.3-1ug/ml, observed in lysates of cell line KELLY on ~150kD bands. Researchers should empirically determine the suitability of the SAP97 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAP97, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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