Rabbit anti-Human, Mouse ALPP/ALPPL2 Polyclonal Antibody | anti-ALPP/ALPPL2 antibody

ALPP/ALPPL2 Polyclonal Antibody

Cat. Num. AAA9703762

• Gene Names: ALPP; ALP; IAP; ALPI; PALP; PLAP; PLAP-1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: ALPP/ALPPL2; Polyclonal Antibody; ALPP/ALPPL2 Polyclonal Antibody; Rabbit Anti-ALPP/ALPPL2 Polyclonal Antibody; ALPP; PLAP; Alkaline phosphatase; placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; ALPPL2; ALPPL; placental-likeALP-1; Alkaline phosphatase Nagao isozyme; Germ cell alkaline phosphatase; GCAP; Placental alkaline phosphatase-like; PLAP-like; anti-ALPP/ALPPL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ALPP/ALPPL2 Polyclonal Antibody detects endogenous levels of ALPP/ALPPL2 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 535

• Applicable Applications for anti-ALPP/ALPPL2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:20000
• Immunogen: Synthesized peptide derived from the N-terminal region of human ALPP/ALPPL2
• Preparation and Storage: Stable for one year at -20 degree C from date of shipment.; For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Western Blot (WB)

(Fig.1. Western Blot analysis of 293 cells using ALPP/ALPPL2 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000.)

Western Blot (WB)

(Fig.2. Western Blot analysis of customer's sample using ALPP/ALPPL2 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000.)

Related Product Information for anti-ALPP/ALPPL2 antibody

Alkaline phosphatase, placental type encoded by ALPP is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 94721246
• NCBI GeneID: 250
• NCBI Accession #: NP_001623.3
• NCBI GenBank Nucleotide #: NP_001623.3
• UniProt Accession #: P05187; P10696
• NCBI Official Full Name: alkaline phosphatase, placental type preproprotein
• NCBI Official Synonym Full Names: alkaline phosphatase, placental
• NCBI Official Symbol: ALPP
• NCBI Official Synonym Symbols: ALP; IAP; ALPI; PALP; PLAP; PLAP-1
• NCBI Protein Information: alkaline phosphatase, placental type
• UniProt Protein Name: Alkaline phosphatase, placental type
• UniProt Gene Name: ALPP
• UniProt Synonym Gene Names: PLAP; PLAP-1
• UniProt Entry Name: PPB1_HUMAN

NCBI Description

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]

Uniprot Description

ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Protein type: Cell surface; Membrane protein, integral; Cofactor and Vitamin Metabolism - folate biosynthesis; Phosphatase (non-protein); Motility/polarity/chemotaxis; Membrane protein, GPI anchor; EC 3.1.3.1

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: cell surface; plasma membrane; integral to membrane

Molecular Function: zinc ion binding; alkaline phosphatase activity; magnesium ion binding

Biological Process: dephosphorylation