Western Blot (WB)
((0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat DLG1 Polyclonal Antibody | anti-DLG1 antibody
Goat anti-DLG1 Antibody
Gene Names
DLG1; hdlg; DLGH1; SAP97; SAP-97; dJ1061C18.1.1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat,
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
DLG1; Polyclonal Antibody; Goat anti-DLG1 Antibody; discs; large homolog 1 (Drosophila); DKFZp761P0818; DKFZp781B0426; DLGH1; SAP97; dJ1061C18.1.1; hdlg; discs large homolog 1; large homolog 1; presynaptic protein SAP97; synapse-associated protein 97; DLG1 antibody; large homolog 1 (Drosophila) antibody; DKFZp761P0818 antibody; DKFZp781B0426 antibody; DLGH1 antibody; SAP97 antibody; dJ1061C18.1.1 antibody; hdlg antibody; discs large homolog 1 antibody; large homolog 1 antibody; presynaptic protein SAP97 antibody; synapse-associated protein 97 antibody; anti-DLG1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat,
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
NSKTRDKGEIPDD
Sequence Length
904
Applicable Applications for anti-DLG1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 150kDa band observed in lysates of cell line KELLY (calculated MW of 103kDa according to NP_004078.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 150kDa band observed in lysates of cell line KELLY (calculated MW of 103kDa according to NP_004078.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-NSKTRDKGEIPDD, from the internal region of the protein sequence according to NP_001091894.1; NP_004078.2.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_001091894.1and NP_004078.2).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,485 Da
NCBI Official Full Name
disks large homolog 1 isoform 1
NCBI Official Synonym Full Names
discs large homolog 1, scribble cell polarity complex component
NCBI Official Symbol
DLG1
NCBI Official Synonym Symbols
hdlg; DLGH1; SAP97; SAP-97; dJ1061C18.1.1
NCBI Protein Information
disks large homolog 1
UniProt Protein Name
Disks large homolog 1
Protein Family
UniProt Gene Name
DLG1
UniProt Synonym Gene Names
SAP-97; SAP97
UniProt Entry Name
DLG1_HUMAN
NCBI Description
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known. [provided by RefSeq, Feb 2011]
Uniprot Description
Essential multidomain scaffolding protein required for normal development (). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.
Research Articles on DLG1
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Product Notes
The DLG1 dlg1 (Catalog #AAA421925) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DLG1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, and may cross-react with other species as described in the data sheet. AAA Biotech's DLG1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 150kDa band observed in lysates of cell line KELLY (calculated MW of 103kDa according to NP_004078.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the DLG1 dlg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NSKTRDKGEI PDD. It is sometimes possible for the material contained within the vial of "DLG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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