Goat ANTXR2 Polyclonal Antibody | anti-ANTXR2 antibody

ANTXR2 (Anthrax Toxin Receptor 2, Capillary Morphogenesis Gene 2 Protein, CMG-2, CMG2)

Cat. Num. AAA647520

• Gene Names: ANTXR2; HFS; ISH; JHF; CMG2; CMG-2
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ANTXR2; Polyclonal Antibody; ANTXR2 (Anthrax Toxin Receptor 2; Capillary Morphogenesis Gene 2 Protein; CMG-2; CMG2); Anti -ANTXR2 (Anthrax Toxin Receptor 2; anti-ANTXR2 antibody

• Host: Goat
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human ANTXR2. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-ANTXR2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:16,000; Western Blot: 0.3-1ug/ml, human, mouse and rat kidney lysates observed on ~60kD bands
• Immunogen: Synthetic peptide corresponding to C-HEGLKLANEQIQK, from ANTX2, at the internal regoin of the protein (NP_477520.2, NP_001139266.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((0.3ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-ANTXR2 antibody

Necessary for cellular interactions with laminin and the extracellular matrix.

Product Categories/Family for anti-ANTXR2 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 224809466
• NCBI GeneID: 118429
• NCBI Accession #: NP_001139266.1
• NCBI GenBank Nucleotide #: NM_001145794.1
• UniProt Accession #: P58335; Q4W5H6; Q59E98; Q5JPE9; Q86UI1; Q8N4J8; Q8NB13; Q96NC7
• Molecular Weight: 53,666 Da
• NCBI Official Full Name: anthrax toxin receptor 2 isoform 2
• NCBI Official Synonym Full Names: anthrax toxin receptor 2
• NCBI Official Symbol: ANTXR2
• NCBI Official Synonym Symbols: HFS; ISH; JHF; CMG2; CMG-2
• NCBI Protein Information: anthrax toxin receptor 2; capillary morphogenesis protein 2; capillary morphogenesis gene 2 protein
• UniProt Protein Name: Anthrax toxin receptor 2
• Protein Family: Anthrax toxin receptor
• UniProt Gene Name: ANTXR2
• UniProt Synonym Gene Names: CMG2; CMG-2
• UniProt Entry Name: ANTR2_HUMAN

NCBI Description

This gene encodes a receptor for anthrax toxin. The protein binds to collagen IV and laminin, suggesting that it may be involved in extracellular matrix adhesion. Mutations in this gene cause juvenile hyaline fibromatosis and infantile systemic hyalinosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

Function: Necessary for cellular interactions with laminin and the extracellular matrix. Ref.1 Ref.15

Subunit structure: Binds laminin, and possibly also collagen type IV. Binds to the protective antigen (PA) of Bacillus anthracis in a divalent cation-dependent manner, with the following preference: calcium > manganese > magnesium > zinc. Binding of PA leads to heptamerization of the receptor-PA complex.

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note: Expressed at the cell surface.Isoform 2: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note: Expressed predominantly within the endoplasmic reticulum and not at the plasma membrane.Isoform 3: Secreted.

Tissue specificity: Expressed in prostate, thymus, ovary, testis, pancreas, colon, heart, kidney, lung, liver, peripheral blood leukocytes, placenta, skeletal muscle, small intestine and spleen. Ref.14

Domain: Binding to PA seems to be effected through the VWA domain.

Involvement in disease: Hyaline fibromatosis syndrome (HFS) [MIM:228600]: An autosomal recessive syndrome characterized by abnormal growth of hyalinized fibrous tissue usually affecting subcutaneous regions on the scalp, ears, neck, face, hands, and feet. The lesions appear as pearly papules or fleshy nodules. Additional features include gingival hypertrophy, progressive joint contractures resulting in severe limitation of mobility, osteopenia, and osteoporosis. Disease severity is variable. Some individuals manifest symptoms in infancy and have additional visceral or systemic involvement. Hyaline deposits in multiple organs, recurrent infections and intractable diarrhea often lead to early death. Surviving children may suffer from severely reduced mobility due to joint contractures. Other patients have later onset of a milder disorder affecting only the face and digits.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.14 Ref.15 Ref.16

Miscellaneous: Upon binding of the protective antigen (PA) of Bacillus anthracis the complex moves to glycosphingolipid-rich lipid rafts, where it is internalized via a clathrin-dependent pathway.

Sequence similarities: Belongs to the ATR family.Contains 1 VWFA domain.

Sequence caution: The sequence AAY40907.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB70976.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAD93150.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.