Western Blot (WB)
(Western blot analysis of extracts from various samples, using NSUN2 antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: hela; )
Rabbit NSUN2 Polyclonal Antibody | anti-NSUN2 antibody
NSUN2 Antibody
Gene Names
NSUN2; MISU; MRT5; SAKI; TRM4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NSUN2; Polyclonal Antibody; NSUN2 Antibody; 5 methycytoisine methyltransferase; D13Wsu123e; FLJ20303; hTrm4; MISU; Myc induced SUN domain containing protein; NOL1/NOP2/Sun domain family 2; NOL1/NOP2/Sun domain family 2 protein; NOL1/NOP2/Sun domain family member 2; NSUN 2; NSUN2_HUMAN; SAKI; Substrate of AIM1/Aurora kinase B; TRM4; tRNA (cytosine 5) methyltransferase; tRNA (cytosine 5) methyltransferase NSUN2; tRNA (cytosine(34)-C(5))-methyltransferase; tRNA (cytosine-5-)-methyltransferase; tRNA methyltransferase 4 homolo; tRNA methyltransferase 4 homolog; anti-NSUN2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NSUN2 antibody detects endogenous levels of NSUN2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
732
Applicable Applications for anti-NSUN2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human NSUN2.
Subcellular Location
Nucleus > Nucleolus. Cytoplasm. Concentrated in the nucleolus during interphase and distributed in the perichromosome and cytoplasm during mitosis.
Tissue Specificity
Expressed in adult and fetal brain and in lymphoblastoid cells.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (92%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (100%), Dog (92%), Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from various samples, using NSUN2 antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: hela; )
Western Blot (WB)
(Western blot analysis of extracts from various samples, using NSUN2 antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: hela; )
Immunohistochemistry (IHC)
(MBS9603331 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603331 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of HEK-293 cells, using NSUN2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HEK-293 cells, using NSUN2 antibody.)
Related Product Information for anti-NSUN2 antibody
Function: RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.
Subunit Structure: Interacts with NPM1 and NCL during interphase; interaction is disrupted following phosphorylation at Ser-139.
Post-translational Modifications: Phosphorylated at Ser-139 by AURKB during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1.
Similarity: Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.
Subunit Structure: Interacts with NPM1 and NCL during interphase; interaction is disrupted following phosphorylation at Ser-139.
Post-translational Modifications: Phosphorylated at Ser-139 by AURKB during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1.
Similarity: Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 90-100 kDa
Predicted: 87 kDa
Predicted: 87 kDa
NCBI Official Full Name
tRNA (cytosine(34)-C(5))-methyltransferase isoform 2
NCBI Official Synonym Full Names
NOP2/Sun RNA methyltransferase family member 2
NCBI Official Symbol
NSUN2
NCBI Official Synonym Symbols
MISU; MRT5; SAKI; TRM4
NCBI Protein Information
tRNA (cytosine(34)-C(5))-methyltransferase
UniProt Protein Name
tRNA (cytosine(34)-C(5))-methyltransferase
Protein Family
UniProt Gene Name
NSUN2
UniProt Synonym Gene Names
SAKI; TRM4; Misu; hTrm4
NCBI Description
This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011]
Uniprot Description
RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.
Research Articles on NSUN2
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Product Notes
The NSUN2 nsun2 (Catalog #AAA9603331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NSUN2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NSUN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NSUN2 nsun2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSUN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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