Mouse RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon) ELISA Kit | RUBCN elisa kit

Mouse RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon) ELISA Kit

Cat. Num. AAA2707790

• Gene Names: RUBCN; SCAR15; RUBICON; KIAA0226
• Reactivity: Mouse
• Synonyms: RUN Domain And Cysteine Rich Domain Containing; Beclin 1 Interacting Protein (Rubicon); Mouse RUN Domain And Cysteine Rich Domain Containing; Beclin 1 Interacting Protein (Rubicon) ELISA Kit; Baron; Rundataxin; Beclin-1 associated RUN domain containing protein; RUBCN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon).; No significant cross-reactivity or interference between RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon) and analogues was observed.
• Sequence Length: 927

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.115ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RUBCN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 224548938
• NCBI GeneID: 9711
• NCBI GenBank Nucleotide #: NP_001139114.1
• UniProt Accession #: Q92622
• NCBI Official Full Name: run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 1
• NCBI Official Synonym Full Names: rubicon autophagy regulator
• NCBI Official Symbol: RUBCN
• NCBI Official Synonym Symbols: SCAR15; RUBICON; KIAA0226
• NCBI Protein Information: run domain Beclin-1-interacting and cysteine-rich domain-containing protein
• UniProt Protein Name: Run domain Beclin-1-interacting and cysteine-rich domain-containing protein
• Protein Family: Run domain Beclin-1-interacting and cysteine-rich domain-containing protein
• UniProt Gene Name: RUBCN
• UniProt Synonym Gene Names: Rubicon; Baron

NCBI Description

The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (PubMed:20974968, PubMed:21062745).