Rabbit Rubicon Polyclonal Antibody | anti-RUBCN antibody

Rubicon Antibody

Cat. Num. AAA9612424

• Gene Names: RUBCN; SCAR15; RUBICON; KIAA0226
• Reactivity: Human; Predicted Reactivity: Bovine(80%), Horse(88%), Rabbit(88%), Dog(88%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Rubicon; Polyclonal Antibody; Rubicon Antibody; Baron; Beclin 1 associated RUN domain containing protein; Beclin-1 associated RUN domain containing protein; hypothetical protein LOC9711; Kiaa0226; OTTHUMP00000208021; OTTHUMP00000208023; OTTHUMP00000208024; RUBIC_HUMAN; RUN domain and cysteine rich domain containing; Beclin 1 interacting protein; Run domain Beclin 1 interacting and cystein rich containing protein; Run domain Beclin-1 interacting and cysteine-rich containing protein; RUN domain protein as Beclin 1 interacting and cysteine rich containing; anti-RUBCN antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Bovine(80%), Horse(88%), Rabbit(88%), Dog(88%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rubicon Antibody detects endogenous levels of total Rubicon.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 927

• Applicable Applications for anti-RUBCN antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Rubicon, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using Rubicon Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-RUBCN antibody

Function: Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (PubMed:20974968, PubMed:21062745).Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA:CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines (PubMed:22423966). Following fungal or viral infection (implicating CLEC7A (dectin-1)-mediated myeloid cell activation or DDX58/RIG-I-dependent sensing of RNA viruses) negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes (PubMed:22423967).

Subcellular Location: Late endosome. Lysosome. Early endosome. Note: Predominantly located in late endosomes/lysosomes, only partially detected in early endosome and not at all in the Golgi apparatus.

Subunit Structure: Associates with PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex II (PI3KC3-C2) in which the core composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 is associated with UVRAG; in the complex interacts directly with PI3KC3 and UVRAG (PubMed:19270696, PubMed:21062745). Interacts with Rab7 (RAB7A or RAB7B) (GTP-bound form); Rab7 and UVRAG compete for RUBCN binding; can interact simultaneously with Rab7 and the PI3K complex (PubMed:20943950, PubMed:20974968, PubMed:21062745). Interacts with CYBA and CYBB; indicative for the association with the CYBA:CYBB NADPH oxidase heterodimer. Interacts with NOX4 and probably associates with the CYBA:NOX4 complex (PubMed:22423966). Interacts with YWHAB and CARD9 in a competetive and stimulation-dependent manner; RUBCN exchanges interaction from YWHAB to CARD9 upon stimulation with beta-1,3-glucan (PubMed:22423967).

NCBI and Uniprot Product Information

• NCBI GI #: 224548938
• NCBI GeneID: 9711
• NCBI Accession #: NP_001139114.1
• NCBI GenBank Nucleotide #: NP_001139114.1
• UniProt Accession #: Q92622
• Molecular Weight: Observed Molecular Weight: (Observed)108kD.; Predicted Molecular Weight: (Calculated)109kDa.
• NCBI Official Full Name: run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 1
• NCBI Official Synonym Full Names: rubicon autophagy regulator
• NCBI Official Symbol: RUBCN
• NCBI Official Synonym Symbols: SCAR15; RUBICON; KIAA0226
• NCBI Protein Information: run domain Beclin-1-interacting and cysteine-rich domain-containing protein
• UniProt Protein Name: Run domain Beclin-1-interacting and cysteine-rich domain-containing protein
• Protein Family: Run domain Beclin-1-interacting and cysteine-rich domain-containing protein
• UniProt Gene Name: RUBCN
• UniProt Synonym Gene Names: Rubicon; Baron

NCBI Description

The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (PubMed:20974968, PubMed:21062745).