Typical Testing Data/Standard Curve (for reference only)
Mouse Interleukin 4 (IL4) ELISA Kit | IL4 elisa kit
Wide-range Interleukin 4 (IL4) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IL4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Secreted, signal peptide; Motility/polarity/chemotaxis; Cytokine; Secreted
Cellular Component: extracellular space; extracellular region; external side of plasma membrane
Molecular Function: growth factor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; interleukin-4 receptor binding
Biological Process: negative regulation of osteoclast differentiation; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-dependent; microglial cell activation; regulation of proton transport; positive regulation of activated T cell proliferation; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; cholesterol metabolic process; negative regulation of chronic inflammatory response; regulation of immune response; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; positive regulation of mast cell degranulation; defense response to protozoan; positive regulation of immunoglobulin production; T-helper 1 cell lineage commitment; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of chemokine biosynthetic process; positive regulation of T cell differentiation; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response in mucosa; positive regulation of B cell activation; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; immune response; positive regulation of protein amino acid phosphorylation; T-helper 2 cell differentiation; positive regulation of defense response to virus by host; negative regulation of transcription, DNA-dependent; negative regulation of T cell activation
Research Articles on IL4
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Product Notes
The Mouse IL4 il4 (Catalog #AAA2709584) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709584 ELISA Kit recognizes Mouse IL4. AAA Biotech's Interleukin 4 (IL4) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the IL4 il4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 4 (IL4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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