Western Blot (WB)
(Western blot analysis of ULK4 in 3T3 cell lysate with ULK4 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Rabbit anti-Human, Mouse ULK4 Polyclonal Antibody | anti-ULK4 antibody
ULK4 Antibody
Gene Names
ULK4; FAM7C1; REC01035
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
ULK4 antibody is affinity chromatography purified via peptide column.
Synonyms
ULK4; Polyclonal Antibody; ULK4 Antibody; FAM7C1; REC01035Unc-51-like kinase 4; unc-51 like kinase 4; anti-ULK4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ULK4 antibody is human and mouse reactive. Multiple isoforms of ULK4 are known to exist.
Purity/Purification
ULK4 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1275
Applicable Applications for anti-ULK4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
ULK4 antibody can be used for detection of ULK4 by Western blot at 0.5 - 1 mug/ml. Antibody can also be used for Immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
ULK4 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human ULK4.
Buffer
ULK4 antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
ULK4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of ULK4 in 3T3 cell lysate with ULK4 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of ULK4 in 3T3 cell lysate with ULK4 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ULK4 in human brain tissue with ULK4 antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ULK4 in human brain tissue with ULK4 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ULK4 in human brain tissue with ULK4 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ULK4 in human brain tissue with ULK4 antibody at 20 μg/mL.)
Related Product Information for anti-ULK4 antibody
ULK4 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK4, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK3, is highly conserved among eukaryotes (3). ULK4 has been implicated as a gene involved in the development of hydrocephalus (3). This neurologic disorder is being used in animal models to elucidate factors responsible for the excessive accumulation of cerebrospinal fluid in hydrocephalic humans (4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 140 kDa
Observed: 165 kDa
Observed: 165 kDa
NCBI Official Full Name
serine/threonine-protein kinase ULK4
NCBI Official Synonym Full Names
unc-51 like kinase 4
NCBI Official Symbol
ULK4
NCBI Official Synonym Symbols
FAM7C1; REC01035
NCBI Protein Information
serine/threonine-protein kinase ULK4; unc-51-like kinase 4
UniProt Protein Name
Serine/threonine-protein kinase ULK4
Protein Family
UniProt Gene Name
ULK4
UniProt Entry Name
ULK4_HUMAN
Uniprot Description
ULK4: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; EC 2.7.11.1; Other group; ULK family
Chromosomal Location of Human Ortholog: 3p22.1
Molecular Function: protein serine/threonine kinase activity; ATP binding
Biological Process: corpus callosum development; ventricular system development; regulation of MAPKKK cascade; microtubule cytoskeleton organization and biogenesis; protein amino acid phosphorylation; regulation of JNK cascade
Research Articles on ULK4
Similar Products
Product Notes
The ULK4 ulk4 (Catalog #AAA153597) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ULK4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ULK4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). ULK4 antibody can be used for detection of ULK4 by Western blot at 0.5 - 1 mug/ml. Antibody can also be used for Immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the ULK4 ulk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ULK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
