Rabbit anti-Human RPS19BP1 Polyclonal Antibody | anti-RPS19BP1 antibody

RPS19BP1 Polyclonal Antibody

Cat. Num. AAA3007222

• Gene Names: RPS19BP1; AROS; S19BP
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RPS19BP1; Polyclonal Antibody; RPS19BP1 Polyclonal Antibody; Active regulator of SIRT1; 40S ribosomal protein S19-binding protein 1; RPS19-binding protein 1; S19BP; AROS; anti-RPS19BP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RPS19BP1 polyclonal antibody detects endogenous levels of RPS19BP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 136

• Applicable Applications for anti-RPS19BP1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to residues in Human RPS19BP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RPS19BP1 pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug) Lane3:EC9706 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug))

Immunoprecipitation (IP)

(Immunoprecipitation of HepG2 cell lysate using RPS19BP1 polyclonal antibody (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 and lane 5). Lane 1 is 30% input. The Western Blot was probed using RPS19BP1 # MBS3007222. “up-arrow” (supernatant) ;“down-arrow (deposition))

Related Product Information for anti-RPS19BP1 antibody

S19BP, also known as RPS19BP1 (ribosomal protein S19 binding protein 1) or AROS, is a 136 amino acid protein that localizes to the nucleolus and belongs to the AROS family. Expressed in a variety of tissues, S19BP interacts with Ribosomal Protein S19 and SIRT1 and is thought to directly regulate SIRT1 function, specifically enhancing the SIRT1-mediated deacetylation of p53 and inhibiting p53-induced transcriptional activity. The gene encoding S19BP maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia.

NCBI and Uniprot Product Information

• NCBI GI #: 34996527
• NCBI GeneID: 91582
• NCBI Accession #: NP_919307.1
• NCBI GenBank Nucleotide #: NP_919307.1
• UniProt Accession #: Q86WX3
• Molecular Weight: ~ 15kDa
• NCBI Official Full Name: active regulator of SIRT1
• NCBI Official Synonym Full Names: ribosomal protein S19 binding protein 1
• NCBI Official Symbol: RPS19BP1
• NCBI Official Synonym Symbols: AROS; S19BP
• NCBI Protein Information: active regulator of SIRT1
• UniProt Protein Name: Active regulator of SIRT1
• Protein Family: Active regulator of SIRT1
• UniProt Gene Name: RPS19BP1
• UniProt Synonym Gene Names: AROS; RPS19-binding protein 1; S19BP
• UniProt Entry Name: AROS_HUMAN

Uniprot Description

RPS19BP1: Direct regulator of SIRT1. Enhances SIRT1-mediated deacetylation of p53/TP53, thereby participating in inhibition of p53/TP53-mediated transcriptional activity. Belongs to the AROS family.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; cytoplasm; nucleolus

Molecular Function: protein binding; enzyme binding