Rabbit MRP-S34 Polyclonal Antibody | anti-MRP-S34 antibody

MRP-S34 (K214) Polyclonal Antibody

Cat. Num. AAA3003641

• Gene Names: MRPS34; MRPS12; COXPD32; MRP-S12; MRP-S34
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MRP-S34; Polyclonal Antibody; MRP-S34 (K214) Polyclonal Antibody; 28S ribosomal protein S34; mitochondrial; S34mt; MRPS34; anti-MRP-S34 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MRP-S34 (K214) polyclonal antibody detects endogenous levels of MRP-S34 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 218

• Applicable Applications for anti-MRP-S34 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 1:10-1:100
• Immunogen: Synthetic peptide, corresponding to amino acids 180-226 of Human MRP-S34.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MRP-S34 (K214) pAb at 1:1000 dilutionLane1:The Kidney tissue lysate of Rat (40ug) Lane2:The Kidney tissue lysate of Mouse (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:Panc1 whole cell lysate (40ug))

Immunoprecipitation (IP)

(Immunoprecipitation of Kidney tissue lysate of Mouse using MRP-S34  (K214)   polyclonal antibody (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 ). Lane 1 is 30% input. The Western Blot was probed using MRP-S34  (K214) # MBS3003641. “up-arrow” (supernatant) ;“down-arrow (deposition))

Related Product Information for anti-MRP-S34 antibody

Mitochondrial ribosomes consist of a large 39S subunit and a small 28S subunit, both of which are comprised of multiple mitochondrial ribosomal proteins (MRPs) that are encoded by nuclear genes and are essential for protein synthesis within mitochondria. MRP-S34 (mitochondrial ribosomal protein S34), is a 218 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. The gene encoding MRP-S34 maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome.

NCBI and Uniprot Product Information

• NCBI GI #: 13027604
• NCBI GeneID: 65993
• NCBI Accession #: NP_076425.1
• NCBI GenBank Nucleotide #: NP_076425.1
• UniProt Accession #: P82930
• Molecular Weight: ~ 26kDa
• NCBI Official Full Name: 28S ribosomal protein S34, mitochondrial isoform 2
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S34
• NCBI Official Symbol: MRPS34
• NCBI Official Synonym Symbols: MRPS12; COXPD32; MRP-S12; MRP-S34
• NCBI Protein Information: 28S ribosomal protein S34, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S34, mitochondrial
• UniProt Gene Name: MRPS34
• UniProt Synonym Gene Names: MRP-S34; S34mt
• UniProt Entry Name: RT34_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

MRPS34: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial inner membrane; ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis