Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with SAP30BP Antibody (Center). MBS9211380 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Rabbit SAP30BP Polyclonal Antibody | anti-SAP30BP antibody
SAP30BP Antibody (Center)
Gene Names
SAP30BP; HTRG; HTRP; HCNGP
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SAP30BP; Polyclonal Antibody; SAP30BP Antibody (Center); SAP30-binding protein; Transcriptional regulator protein HCNGP; SAP30BP {ECO:0000312|EMBL:AAH075921}; anti-SAP30BP antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SAP30BP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-188 amino acids from the Central region of human SAP30BP.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
160-188
Sequence Length
308
Applicable Applications for anti-SAP30BP antibody
Immunofluorescence (IF), ELISA (EIA), Western Blot (WB)
Application Notes
IF~~1:25
WB~~1:1000
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with SAP30BP Antibody (Center). MBS9211380 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with SAP30BP Antibody (Center). MBS9211380 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Western Blot (WB)
(SAP30BP Antibody (Center) western blot analysis in WiDr cell line and human placenta tissue lysates (35ug/lane).This demonstrates the SAP30BP antibody detected the SAP30BP protein (arrow).)
Western Blot (WB)
(SAP30BP Antibody (Center) western blot analysis in WiDr cell line and human placenta tissue lysates (35ug/lane).This demonstrates the SAP30BP antibody detected the SAP30BP protein (arrow).)
Related Product Information for anti-SAP30BP antibody
Induces cell death. May act as a transcriptional corepressor of a gene related to cell survival. May be involved in the regulation of beta-2-microglobulin genes.
Product Categories/Family for anti-SAP30BP antibody
References
Li J.-F., et al. J. Biochem. 136:169-176(2004).
Gou D., et al. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
Ding J.B., et al. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Hu R.-M., et al. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Ota T., et al. Nat. Genet. 36:40-45(2004).
Gou D., et al. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
Ding J.B., et al. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Hu R.-M., et al. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Ota T., et al. Nat. Genet. 36:40-45(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33870
NCBI Official Full Name
SAP30-binding protein isoform 2
NCBI Official Synonym Full Names
SAP30 binding protein
NCBI Official Symbol
SAP30BP
NCBI Official Synonym Symbols
HTRG; HTRP; HCNGP
NCBI Protein Information
SAP30-binding protein
UniProt Protein Name
SAP30-binding protein
Protein Family
UniProt Gene Name
SAP30BP
UniProt Entry Name
S30BP_HUMAN
Uniprot Description
SAP30BP: Induces cell death. May act as a transcriptional corepressor of a gene related to cell survival. May be involved in the regulation of beta-2-microglobulin genes. Belongs to the HCNGP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: nucleoplasm; intermediate filament cytoskeleton; nucleus
Molecular Function: protein binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; negative regulation of gene expression, epigenetic; gene expression; regulation of gene expression, epigenetic
Research Articles on SAP30BP
Similar Products
Product Notes
The SAP30BP sap30bp (Catalog #AAA9211380) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 160-188. The SAP30BP Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's SAP30BP can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the SAP30BP sap30bp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAP30BP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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