Rabbit PAK4/5/6 Polyclonal Antibody | anti-PAK4/5/6 antibody

PAK4/5/6 (V469) Polyclonal Antibody

Cat. Num. AAA3003555

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PAK4/5/6; Polyclonal Antibody; PAK4/5/6 (V469) Polyclonal Antibody; Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4; PAK4; KIAA1142; Serine/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6; PAK-6; PAK6; PAK5; Serine/threonine-protein kinase PAK 7; p21-activated kinase 5; p21-activated kinase 7; PAK-7; PAK7; KIAA1264; anti-PAK4/5/6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PAK4/5/6 (V469) polyclonal antibody detects endogenous levels of PAK4, PAK5 and PAK6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 591

• Applicable Applications for anti-PAK4/5/6 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 1:10-1:100
• Immunogen: Synthetic peptide, corresponding to amino acids 441-490 of Human PAK4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PAK4/5/6 (V469) pAb at 1:500 dilutionLane1:H1792 whole cell lysate (20ug) Lane2:MCF-7 whole cell lysate (20ug) Lane3:PC3 whole cell lysate (20ug) Lane4:The Testis tissue lysate of Mouse (40ug) Lane5:The Testis tissue lysate of Rat (40ug))

Immunoprecipitation (IP)

(Immunoprecipitation of HEK293T cell lysate using PAK4/5/6  (V469) polyclonal antibody (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 ). Lane 1 is 30% input. The Western Blot was probed using PAK4/5/6  (V469) # MBS3003555. “up-arrow” (supernatant) ;“down-arrow (deposition))

Western Blot (WB)

(Western Blot (WB) analysis of PAK4/5/6 (V469) pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:The Heart tissue lysate of Mouse (40ug) Lane4:H9C2 whole cell lysate (40ug))

Related Product Information for anti-PAK4/5/6 antibody

PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction.PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen.

NCBI and Uniprot Product Information

• NCBI GI #: 62422554
• NCBI GeneID: 10298
• NCBI Accession #: NP_001014831.1
• NCBI GenBank Nucleotide #: NP_001014831.1
• UniProt Accession #: O96013; Q9NQU5; Q9P286
• Molecular Weight: ~ 64kDa (PAK4), 80kDa (PAK5), 75kDa (PAK6)
• NCBI Official Full Name: serine/threonine-protein kinase PAK 4 isoform 1
• NCBI Official Synonym Full Names: p21 (RAC1) activated kinase 4
• NCBI Official Symbol: PAK4
• NCBI Protein Information: serine/threonine-protein kinase PAK 4
• UniProt Protein Name: Serine/threonine-protein kinase PAK 4
• UniProt Gene Name: PAK4
• UniProt Synonym Gene Names: KIAA1142; PAK-4
• UniProt Entry Name: PAK4_HUMAN

NCBI Description

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; EC 2.7.11.1; STE group; STE20 family; PAKB subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: Golgi apparatus; focal adhesion

Molecular Function: protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: cell proliferation; cell migration; apoptosis; cytoskeleton organization and biogenesis; mitotic cell cycle; cell motility; cell growth; signal transduction; protein amino acid phosphorylation