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Testing Data (Sample(30 ug of whole cell lysate)A: HeLa S3 B: Hep G2 C: MOLT415% SDS PAGEPrimary antibody diluted at 1: 1000)

Rabbit anti-Human RPA 14 kDa subunit Polyclonal Antibody | anti-RPA3 antibody

RPA 14 kDa subunit antibody

Gene Names
RPA3; REPA3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified by antigen-affinity chromatography.
Synonyms
RPA 14 kDa subunit; Polyclonal Antibody; RPA 14 kDa subunit antibody; anti-RPA3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified by antigen-affinity chromatography.
Form/Format
Supplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
Sequence Length
121
Applicable Applications for anti-RPA3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunogen Type
Recombinant protein
Immunogen Description
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 91 of RPA 14 kDa subunit
Target Name
RPA 14 kDa subunit
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample(30 ug of whole cell lysate)A: HeLa S3 B: Hep G2 C: MOLT415% SDS PAGEPrimary antibody diluted at 1: 1000)

Testing Data (Sample(30 ug of whole cell lysate)A: HeLa S3 B: Hep G2 C: MOLT415% SDS PAGEPrimary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded lung CA patient tumor, using RPA 14 kDa subunit antibody at 1: 100 dilution.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded lung CA patient tumor, using RPA 14 kDa subunit antibody at 1: 100 dilution.)
Related Product Information for anti-RPA3 antibody
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Product Categories/Family for anti-RPA3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,569 Da
NCBI Official Full Name
replication protein A 14 kDa subunit
NCBI Official Synonym Full Names
replication protein A3, 14kDa
NCBI Official Symbol
RPA3
NCBI Official Synonym Symbols
REPA3
NCBI Protein Information
replication protein A 14 kDa subunit
UniProt Protein Name
Replication protein A 14 kDa subunit
Protein Family
UniProt Gene Name
RPA3
UniProt Synonym Gene Names
REPA3; RPA14; RP-A p14; RF-A protein 3
UniProt Entry Name
RFA3_HUMAN

Uniprot Description

RPA3: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: nucleoplasm; DNA replication factor A complex

Molecular Function: protein binding; damaged DNA binding; single-stranded DNA binding

Biological Process: mismatch repair; regulation of mitotic cell cycle; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; regulation of cell proliferation; telomere maintenance via semi-conservative replication; DNA recombinase assembly; base-excision repair; nucleotide-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle

Research Articles on RPA3

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Product Notes

The RPA3 rpa3 (Catalog #AAA9407438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPA 14 kDa subunit antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPA 14 kDa subunit can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:500-1:3000 Immunohistochemistry: 1:100-1:250. Researchers should empirically determine the suitability of the RPA3 rpa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA 14 kDa subunit, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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