Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded SCC25 xenograft using MAN1B1 antibody at a dilution of 1:100)
Rabbit MAN1B1 Polyclonal Antibody | anti-MAN1B1 antibody
MAN1B1 antibody
WB: 1:500-1:3000
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded SCC25 xenograft using MAN1B1 antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded SCC25 xenograft using MAN1B1 antibody at a dilution of 1:100)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5 % SDS PAGE gel and MAN1B1 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5 % SDS PAGE gel and MAN1B1 antibody at a dilution of 1:1000)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Dec 2011]
Uniprot Description
MAN1B1: Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man(5-6)GlcNAc(2). Defects in MAN1B1 are the cause of mental retardation autosomal recessive type 15 (MRT15). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the glycosyl hydrolase 47 family.
Protein type: Endoplasmic reticulum; Glycan Metabolism - N-glycan biosynthesis; EC 3.2.1.113; Hydrolase; Calcium-binding; Membrane protein, integral
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; vesicle
Molecular Function: mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; calcium ion binding
Biological Process: ER-associated protein catabolic process; cellular protein metabolic process; protein folding; protein amino acid N-linked glycosylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; oligosaccharide metabolic process
Disease: Mental Retardation, Autosomal Recessive 15
Research Articles on MAN1B1
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Product Notes
The MAN1B1 man1b1 (Catalog #AAA835226) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MAN1B1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:100-1:250 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the MAN1B1 man1b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAN1B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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