Rabbit ADRBK1 Antibody | anti-ADRBK1 antibody

ADRBK1 Antibody

Cat. Num. AAA8500586

• Gene Names: GRK2; BARK1; ADRBK1; BETA-ARK1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: ADRBK1; ADRBK1 Antibody; BARK1; BETA-ARK1; FLJ16718; GRK2; anti-ADRBK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 689

• Applicable Applications for anti-ADRBK1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human ADRBK1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

(Western blot analysis of extracts of Jurkatcell lines, using ADRBK1 antibody.)

Related Product Information for anti-ADRBK1 antibody

G-protein-coupled receptor kinase 2 (GRK2), also known as beta-adrenergic receptor kinase 1 (beta-ARK1), is a member of the GRK family, which phosphorylates the activated form of G-protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR (1). GRK2 kinase activity and cellular localization are tightly regulated by interactions with activated receptors, G-beta and G-gamma subunits, adaptor proteins, phospholipids, caveolin and calmodulin, as well as by phosphorylation (1). PKC phosphorylation enhances GRK2 activity by promoting its membrane localization and by abolishing the inhibitory association of calmodulin (2, 3). PKA phosphorylates GRK2 at Ser685, which facilitates the association of GRK2 with a beta-adrenergic receptor (4). Erk inhibits GRK2 activity via phosphorylation at Ser670 (5). Src phosphorylates GRK2 at multiple tyrosine residues (Tyr13, 86 and 92), which activates GRK2 activity and promotes GRK2 degradation (6, 7).

Product Categories/Family for anti-ADRBK1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Pitcher, J. A. et al. (1998) Annu. Rev. Biochem. 67, 653-692. 2. Winstel, R. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 2105-2109. 3. Krasel, C. et al. (2001) J. Biol. Chem. 276, 1911-1915. 4. Cong, M. et al. (2001) J. Biol. Chem. 276, 15192-15199. 5. Pitcher, J. A. et al. (1999) J. Biol. Chem. 274, 34531-34534. 6. Sarnago, S. et al. (1999) J. Biol. Chem. 274, 34411-34416. 7. Penela, P. et al. (2001) EMBO J. 20, 5129-5138.

NCBI and Uniprot Product Information

• NCBI GI #: 148539876
• NCBI GeneID: 156
• NCBI Accession #: NP_001610.2
• NCBI GenBank Nucleotide #: NM_001619.4
• UniProt Accession #: P25098; Q13837; Q6GTT3; B0ZBE1
• Molecular Weight: 80kDa
• NCBI Official Full Name: beta-adrenergic receptor kinase 1
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 2
• NCBI Official Symbol: GRK2
• NCBI Official Synonym Symbols: BARK1; ADRBK1; BETA-ARK1
• NCBI Protein Information: beta-adrenergic receptor kinase 1
• UniProt Protein Name: Beta-adrenergic receptor kinase 1
• UniProt Gene Name: GRK2
• UniProt Synonym Gene Names: Beta-ARK-1

NCBI Description

This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]

Uniprot Description

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner (PubMed:19306925, PubMed:19715378). Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by faciltating the trafficking of SMO into the cilium and the stimulation of SMO activity ().