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Western Blot (WB) (Western blotAll lanes: Replication protein A 14 kDasubunit antibody at 2ug/mlLane 1: LO2 whole cell lysateLane 2:HepG2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 14kDaObserved band size: 14kDa)

Rabbit anti-Human Replication protein A 14 kDa subunit Polyclonal Antibody | anti-RPA3 antibody

Rabbit anti-human Replication protein A 14 kDa subunit polyclonal Antibody

Gene Names
RPA3; REPA3; RP-A p14
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Synonyms
Replication protein A 14 kDa subunit; Polyclonal Antibody; Rabbit anti-human Replication protein A 14 kDa subunit polyclonal Antibody; Replication factor A protein 3; RPA3; REPA3; RPA14; anti-RPA3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Sequence Length
121
Applicable Applications for anti-RPA3 antibody
ELISA (EIA), Western Blot (WB)
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human Replication protein A 14 kDa subunit protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30409 / sc-46623 / sc-46507 / sc-30411
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: Replication protein A 14 kDasubunit antibody at 2ug/mlLane 1: LO2 whole cell lysateLane 2:HepG2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 14kDaObserved band size: 14kDa)

Western Blot (WB) (Western blotAll lanes: Replication protein A 14 kDasubunit antibody at 2ug/mlLane 1: LO2 whole cell lysateLane 2:HepG2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 14kDaObserved band size: 14kDa)
Related Product Information for anti-RPA3 antibody
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Ref.7 Ref.9 Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,569 Da
NCBI Official Full Name
replication protein A 14 kDa subunit
NCBI Official Synonym Full Names
replication protein A3
NCBI Official Symbol
RPA3
NCBI Official Synonym Symbols
REPA3; RP-A p14
NCBI Protein Information
replication protein A 14 kDa subunit
UniProt Protein Name
Replication protein A 14 kDa subunit
Protein Family
UniProt Gene Name
RPA3
UniProt Synonym Gene Names
REPA3; RPA14; RP-A p14; RF-A protein 3
UniProt Entry Name
RFA3_HUMAN

Uniprot Description

RPA3: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: DNA replication factor A complex; nucleoplasm

Molecular Function: damaged DNA binding; protein binding; single-stranded DNA binding

Biological Process: base-excision repair; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA repair; DNA replication; DNA strand elongation during DNA replication; double-strand break repair; double-strand break repair via homologous recombination; error-prone postreplication DNA repair; G1/S transition of mitotic cell cycle; mismatch repair; mitotic cell cycle; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; regulation of cell proliferation; regulation of mitotic cell cycle; telomere maintenance; telomere maintenance via recombination; telomere maintenance via semi-conservative replication; transcription-coupled nucleotide-excision repair

Research Articles on RPA3

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Product Notes

The RPA3 rpa3 (Catalog #AAA1490173) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Replication protein A 14 kDa subunit polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Replication protein A 14 kDa subunit can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the RPA3 rpa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Replication protein A 14 kDa subunit, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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