Rabbit RibosomalL31 Polyclonal Antibody | anti-L102 antibody

Ribosomal Protein L31 (L102) polyclonal antibody

Cat. Num. AAA3014026

• Gene Names: RPL31; L31
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RibosomalL31; Polyclonal Antibody; Ribosomal Protein L31 (L102) polyclonal antibody; 60S ribosomal protein L31; Large ribosomal subunit protein El31; RPL31; anti-L102 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ribosomal Protein L31 (L102) polyclonal antibody endogenous levels of Ribosomal Protein L31.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 125

• Applicable Applications for anti-L102 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 40-162 of Ribosomal Protein L31 (L102)
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Ribosomal Protein L31 (L102) polyclonal antibody at 1:500 dilutionLane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:The Testis tissue lysate of Rat (40ug) Lane5:The Testis tissue lysate of Mouse (40ug))

Related Product Information for anti-L102 antibody

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation. Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein 

NCBI and Uniprot Product Information

• NCBI GI #: 4506633
• NCBI GeneID: 6160
• NCBI Accession #: NP_000984.1
• NCBI GenBank Nucleotide #: NP_000984.1
• UniProt Accession #: P62899
• Molecular Weight: ~ 14kDa
• NCBI Official Full Name: 60S ribosomal protein L31 isoform 1
• NCBI Official Synonym Full Names: ribosomal protein L31
• NCBI Official Symbol: RPL31
• NCBI Official Synonym Symbols: L31
• NCBI Protein Information: 60S ribosomal protein L31
• UniProt Protein Name: 60S ribosomal protein L31
• UniProt Gene Name: RPL31
• UniProt Entry Name: RL31_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L31E family of ribosomal proteins. It is located in the cytoplasm. Higher levels of expression of this gene in familial adenomatous polyps compared to matched normal tissues have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL31: a ribosomal protein of the L31E family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Higher levels of expression in familial adenomatous polyps compared to matched normal tissues have been observed.

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: focal adhesion; membrane; cytosol

Molecular Function: structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; translation; viral reproduction; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; viral infectious cycle; translational termination