Rabbit RPL7 Polyclonal Antibody | anti-RPL7 antibody

RPL7 (N248) Polyclonal Antibody

Cat. Num. AAA3004310

• Gene Names: RPL7; L7; humL7-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RPL7; Polyclonal Antibody; RPL7 (N248) Polyclonal Antibody; 60S ribosomal protein L7; anti-RPL7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ribosomal Protein L7 (N248) polyclonal antibody detects endogenous levels of Ribosomal Protein L7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 248

• Applicable Applications for anti-RPL7 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IP: 1:10-1:100
• Immunogen: Synthetic peptide, corresponding to amino acids 220-270 of Human Ribosomal Protein L7.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RPL7 (N248) polyclonal antibody at 1:500 dilutionLane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:The Spleen tissue lysate of Rat (40ug) Lane5:The Spleen tissue lysate of Mouse (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of RPL7 (N248) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Immunoprecipitation (IP)

(Immunoprecipitation of HEK293T cell lysate using RPL7  (N248) pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 and lane 5). Lane 1 is 30% input. The Western Blot was probed using RPL7 (N248) # MBS3004310.“up-arrow” (supernatant) ; “down-arrow (deposition))

Western Blot (WB)

(Western Blot (WB) analysis of RPL7 (N248) pAb at 1:5000 dilutionLane1:HCT116 whole cell lysate (10ug) Lane2:MCF-7 whole cell lysate (10ug) Lane3:The Brain tissue lysate of Mouse (10ug) Lane4:The Brain tissue lysate of Rat (10ug))

Related Product Information for anti-RPL7 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

NCBI and Uniprot Product Information

• NCBI GI #: 15431301
• NCBI GeneID: 6129
• NCBI Accession #: NP_000962.2
• NCBI GenBank Nucleotide #: NP_000962.2
• UniProt Accession #: P18124
• Molecular Weight: ~ 29kDa
• NCBI Official Full Name: 60S ribosomal protein L7 isoform 1
• NCBI Official Synonym Full Names: ribosomal protein L7
• NCBI Official Symbol: RPL7
• NCBI Official Synonym Symbols: L7; humL7-1
• NCBI Protein Information: 60S ribosomal protein L7
• UniProt Protein Name: 60S ribosomal protein L7
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL7
• UniProt Entry Name: RL7_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL7: a ribosomal protein of the L30P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. Can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus.

Protein type: RNA processing; Nuclear receptor co-regulator; Ribosomal

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: focal adhesion; membrane; cytoplasm; nucleolus; cytosol; ribonucleoprotein complex; nucleus

Molecular Function: mRNA binding; protein binding; protein homodimerization activity; structural constituent of ribosome; DNA binding; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translation; viral reproduction; translational termination; ribosomal large subunit biogenesis and assembly; viral infectious cycle; cellular protein metabolic process; translational elongation; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; rRNA processing