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Western Blot (WB)
(Western Blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using MBS633477.)
Rabbit Ribosomal Protein S6, Monoclonal Antibody | anti-Despr_1827 antibody
Ribosomal Protein S6, (BSA, Azide & Glycerol Free)
Reactivity
Human, Monkey, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
SupernatantSupernatant
Synonyms
Ribosomal Protein S6; ; Monoclonal Antibody; (BSA; Azide & Glycerol Free); Anti -Ribosomal Protein S6; anti-Despr_1827 antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
4i8
Specificity
Recognizes endogenous levels of total human S6 ribosomal protein independent of phosphorylation. MW
Purity/Purification
Supernatant
Supernatant
Supernatant
Form/Format
Supplied in liquid with PBS. Also available with BSA, azide and glycerol. See R2031-30D.
Applicable Applications for anti-Despr_1827 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:100. Heating in citrate buffer required.
Immunofluorescence: 1:100. Cell smears only.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:100. Heating in citrate buffer required.
Immunofluorescence: 1:100. Cell smears only.
Immunogen
Synthetic peptide corresponding to residues 185-205 of human S6 ribosomal protein (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Note: We do not recommended adding glycerol as a cryopreservative.
Western Blot (WB)
(Western Blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using MBS633477.)
Western Blot (WB)
(Western Blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using MBS633477.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic localization, using MBS633477.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic localization, using MBS633477.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MBS633477.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MBS633477.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using MBS633477.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using MBS633477.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using MBS633477.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using MBS633477.)
Related Product Information for anti-Despr_1827 antibody
To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, have been identified and are located within a small 19 amino acid region in the S6 carboxy terminus (4,5).
Product Categories/Family for anti-Despr_1827 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Despr_1827 (Catalog #AAA633477) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ribosomal Protein S6, (BSA, Azide & Glycerol Free) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ribosomal Protein S6, can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:100. Heating in citrate buffer required. Immunofluorescence: 1:100. Cell smears only. Researchers should empirically determine the suitability of the Despr_1827 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribosomal Protein S6,, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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